Aegilops sharonensis genome-assisted identification of stem rust resistance gene Sr62

Guotai Yu, Oadi Matny, Nicolas Champouret, Burkhard Steuernagel, Matthew J. Moscou, Inmaculada Hernández-Pinzón, Phon Green, Sadiye Hayta, Mark Smedley, Wendy Harwood, Ngonidzashe Kangara, Yajuan Yue, Catherine Gardener, Mark J. Banfield, Pablo D. Olivera, Cole Welchin, Jamie Simmons, Eitan Millet, Anna Minz-Dub, Moshe RonenRaz Avni, Amir Sharon, Mehran Patpour, Annemarie F. Justesen, Murukarthick Jayakodi, Axel Himmelbach, Nils Stein, Shuangye Wu, Jesse Poland, Jennifer Ens, Curtis Pozniak, Miroslava Karafiátová, István Molnár, Jaroslav Doležel, Eric R. Ward, T. Lynne Reuber, Jonathan D.G. Jones, Martin Mascher, Brian J. Steffenson, Brande B.H. Wulff

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The wild relatives and progenitors of wheat have been widely used as sources of disease resistance (R) genes. Molecular identification and characterization of these R genes facilitates their manipulation and tracking in breeding programmes. Here, we develop a reference-quality genome assembly of the wild diploid wheat relative Aegilops sharonensis and use positional mapping, mutagenesis, RNA-Seq and transgenesis to identify the stem rust resistance gene Sr62, which has also been transferred to common wheat. This gene encodes a tandem kinase, homologues of which exist across multiple taxa in the plant kingdom. Stable Sr62 transgenic wheat lines show high levels of resistance against diverse isolates of the stem rust pathogen, highlighting the utility of Sr62 for deployment as part of a polygenic stack to maximize the durability of stem rust resistance.

Original languageEnglish (US)
Article number1607
JournalNature communications
Volume13
Issue number1
DOIs
StatePublished - Dec 2022

Bibliographical note

Funding Information:
We are grateful to the Harold and Adele Lieberman Germplasm Bank (Tel Aviv University), Hazera Seeds Ltd and Limagrain for making germplasm available. We thank Ryan Johnson for phenotyping some of the Sr62 introgression line mutants, Yue Jin for supplying several of the stem rust cultures, JIC Horticultural Services for plant husbandry, Matthew Heaton for assistance with figure design, Manuela Knauft and Ines Walde for technical assistance on Hi-C library preparation and sequencing, Anne Fiebig for sequence data submission, Jan Vrána, Zdeňka Dubská and Romana Šperková for assistance with chromosome sorting and DNA amplification, Bob McIntosh for review of the draft manuscript, and Willem Boshoff for helpful discussion. This research was supported by the NBI Computing Infrastructure for Science (CiS) group and financed by grants from the 2Blades Foundation, USA, to B.J.S. and B.B.H.W.; the Biotechnology and Biological Sciences Research Council (BBSRC) Designing Future Wheat Cross-Institute Strategic Programme to B.B.H.W. (BBS/E/J/000PR9780); the Lieberman-Okinow Endowment at the University of Minnesota to B.J.S.; Human Frontier Science Program long-term fellowship (LT000218/2011-L) to M.J.M.; the Gordon and Betty Moore Foundation through grant GBMF4725 to the 2Blades Foundation; and the Gatsby Charitable Foundation to J.D.G.J.

Funding Information:
We are grateful to the Harold and Adele Lieberman Germplasm Bank (Tel Aviv University), Hazera Seeds Ltd and Limagrain for making germplasm available. We thank Ryan Johnson for phenotyping some of the Sr62 introgression line mutants, Yue Jin for supplying several of the stem rust cultures,?JIC Horticultural Services for plant husbandry, Matthew Heaton for assistance with figure design, Manuela Knauft and Ines Walde for technical assistance on Hi-C library preparation and sequencing, Anne Fiebig for sequence data submission, Jan Vr?na, Zde?ka Dubsk? and Romana ?perkov? for assistance with chromosome sorting and DNA amplification, Bob McIntosh for review of the draft manuscript, and Willem Boshoff for helpful discussion. This research was supported by the NBI Computing Infrastructure for Science (CiS) group and financed by grants from the 2Blades Foundation, USA, to B.J.S. and B.B.H.W.; the Biotechnology and Biological Sciences Research Council (BBSRC) Designing Future Wheat Cross-Institute Strategic Programme to B.B.H.W. (BBS/E/J/000PR9780); the Lieberman-Okinow Endowment at the University of Minnesota to B.J.S.; Human Frontier Science Program long-term fellowship (LT000218/2011-L) to M.J.M.; the Gordon and Betty Moore Foundation through grant GBMF4725 to the 2Blades Foundation; and the Gatsby Charitable Foundation to J.D.G.J.

Publisher Copyright:
© 2022, The Author(s).

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't

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