Advancing crop transformation in the era of genome editing

Fredy Altpeter, Nathan M. Springer, Laura E. Bartley, Ann E. Blechl, Thomas P. Brutnell, Vitaly Citovsky, Liza J. Conrad, Stanton B. Gelvin, David P. Jackson, Albert P. Kausch, Peggy G. Lemaux, June I. Medford, Martha L. Orozco-Cárdenas, David M. Tricoli, Joyce Van Eck, Daniel F. Voytas, Virginia Walbot, Kan Wang, Zhanyuan J. Zhang, C. Neal Stewart

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

Plant transformation has enabled fundamental insights into plant biology and revolutionized commercial agriculture. Unfortunately, for most crops, transformation and regeneration remain arduous even after more than 30 years of technological advances. Genome editing provides novel opportunities to enhance crop productivity but relies on genetic transformation and plant regeneration, which are bottlenecks in the process. Here, we review the state of plant transformation and point to innovations needed to enable genome editing in crops. Plant tissue culture methods need optimization and simplification for efficiency and minimization of time in culture. Currently, specialized facilities exist for crop transformation. Single-cell and robotic techniques should be developed for high-throughput genomic screens. Plant genes involved in developmental reprogramming, wound response, and/or homologous recombination should be used to boost the recovery of transformed plants. Engineering universal Agrobacterium tumefaciens strains and recruiting other microbes, such as Ensifer or Rhizobium, could facilitate delivery of DNA and proteins into plant cells. Synthetic biology should be employed for de novo design of transformation systems. Genome editing is a potential game-changer in crop genetics when plant transformation systems are optimized.

Original languageEnglish (US)
Pages (from-to)1510-1520
Number of pages11
JournalPlant Cell
Volume28
Issue number7
DOIs
StatePublished - Jul 2016

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genome
crops
Ensifer
synthetic biology
Regeneration
plant genetics
homologous recombination
plant biology
plant damage
genetic transformation
plant proteins
Agrobacterium radiobacter
Synthetic Biology
Rhizobium
Genetic Transformation
Plant Genes
Agrobacterium tumefaciens
tissue culture
plant tissues
engineering

Cite this

Altpeter, F., Springer, N. M., Bartley, L. E., Blechl, A. E., Brutnell, T. P., Citovsky, V., ... Neal Stewart, C. (2016). Advancing crop transformation in the era of genome editing. Plant Cell, 28(7), 1510-1520. https://doi.org/10.1105/tpc.16.00196

Advancing crop transformation in the era of genome editing. / Altpeter, Fredy; Springer, Nathan M.; Bartley, Laura E.; Blechl, Ann E.; Brutnell, Thomas P.; Citovsky, Vitaly; Conrad, Liza J.; Gelvin, Stanton B.; Jackson, David P.; Kausch, Albert P.; Lemaux, Peggy G.; Medford, June I.; Orozco-Cárdenas, Martha L.; Tricoli, David M.; Van Eck, Joyce; Voytas, Daniel F.; Walbot, Virginia; Wang, Kan; Zhang, Zhanyuan J.; Neal Stewart, C.

In: Plant Cell, Vol. 28, No. 7, 07.2016, p. 1510-1520.

Research output: Contribution to journalArticle

Altpeter, F, Springer, NM, Bartley, LE, Blechl, AE, Brutnell, TP, Citovsky, V, Conrad, LJ, Gelvin, SB, Jackson, DP, Kausch, AP, Lemaux, PG, Medford, JI, Orozco-Cárdenas, ML, Tricoli, DM, Van Eck, J, Voytas, DF, Walbot, V, Wang, K, Zhang, ZJ & Neal Stewart, C 2016, 'Advancing crop transformation in the era of genome editing', Plant Cell, vol. 28, no. 7, pp. 1510-1520. https://doi.org/10.1105/tpc.16.00196
Altpeter F, Springer NM, Bartley LE, Blechl AE, Brutnell TP, Citovsky V et al. Advancing crop transformation in the era of genome editing. Plant Cell. 2016 Jul;28(7):1510-1520. https://doi.org/10.1105/tpc.16.00196
Altpeter, Fredy ; Springer, Nathan M. ; Bartley, Laura E. ; Blechl, Ann E. ; Brutnell, Thomas P. ; Citovsky, Vitaly ; Conrad, Liza J. ; Gelvin, Stanton B. ; Jackson, David P. ; Kausch, Albert P. ; Lemaux, Peggy G. ; Medford, June I. ; Orozco-Cárdenas, Martha L. ; Tricoli, David M. ; Van Eck, Joyce ; Voytas, Daniel F. ; Walbot, Virginia ; Wang, Kan ; Zhang, Zhanyuan J. ; Neal Stewart, C. / Advancing crop transformation in the era of genome editing. In: Plant Cell. 2016 ; Vol. 28, No. 7. pp. 1510-1520.
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