Advances in the directed evolution of proteins

Michael D. Lane, Burckhard Seelig

Research output: Contribution to journalReview articlepeer-review

79 Scopus citations


Natural evolution has produced a great diversity of proteins that can be harnessed for numerous applications in biotechnology and pharmaceutical science. Commonly, specific applications require proteins to be tailored by protein engineering. Directed evolution is a type of protein engineering that yields proteins with the desired properties under well-defined conditions and in a practical time frame. While directed evolution has been employed for decades, recent creative developments enable the generation of proteins with previously inaccessible properties. Novel selection strategies, faster techniques, the inclusion of unnatural amino acids or modifications, and the symbiosis of rational design approaches and directed evolution continue to advance protein engineering.

Original languageEnglish (US)
Pages (from-to)129-136
Number of pages8
JournalCurrent opinion in chemical biology
StatePublished - Oct 1 2014

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Publisher Copyright:
© 2014 Elsevier Ltd.


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