Advanced single voxel 1H magnetic resonance spectroscopy techniques in humans: Experts' consensus recommendations

The Experts' Working Group on Advanced Single Voxel H MRS, Gülin Öz, Dinesh K. Deelchand, Jannie P. Wijnen, Vladimír Mlynárik, Lijing Xin, Ralf Mekle, Ralph Noeske, Tom W.J. Scheenen, Ivan Tkáč

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80 Scopus citations


Conventional proton MRS has been successfully utilized to noninvasively assess tissue biochemistry in conditions that result in large changes in metabolite levels. For more challenging applications, namely, in conditions which result in subtle metabolite changes, the limitations of vendor-provided MRS protocols are increasingly recognized, especially when used at high fields (≥3 T) where chemical shift displacement errors, B0 and B1 inhomogeneities and limitations in the transmit B1 field become prominent. To overcome the limitations of conventional MRS protocols at 3 and 7 T, the use of advanced MRS methodology, including pulse sequences and adjustment procedures, is recommended. Specifically, the semiadiabatic LASER sequence is recommended when TE values of 25-30 ms are acceptable, and the semiadiabatic SPECIAL sequence is suggested as an alternative when shorter TE values are critical. The magnetic field B0 homogeneity should be optimized and RF pulses should be calibrated for each voxel. Unsuppressed water signal should be acquired for eddy current correction and preferably also for metabolite quantification. Metabolite and water data should be saved in single shots to facilitate phase and frequency alignment and to exclude motion-corrupted shots. Final averaged spectra should be evaluated for SNR, linewidth, water suppression efficiency and the presence of unwanted coherences. Spectra that do not fit predefined quality criteria should be excluded from further analysis. Commercially available tools to acquire all data in consistent anatomical locations are recommended for voxel prescriptions, in particular in longitudinal studies. To enable the larger MRS community to take advantage of these advanced methods, a list of resources for these advanced protocols on the major clinical platforms is provided. Finally, a set of recommendations are provided for vendors to enable development of advanced MRS on standard platforms, including implementation of advanced localization sequences, tools for quality assurance on the scanner, and tools for prospective volume tracking and dynamic linear shim corrections.

Original languageEnglish (US)
Article numbere4236
JournalNMR in biomedicine
Issue number5
Early online dateJan 10 2020
StatePublished - 2020

Bibliographical note

Funding Information:
The preparation of this manuscript was supported by the National Institute of Neurological Disorders and Stroke (NINDS) grant R01 NS080816. The Center for Magnetic Resonance Research is supported by the National Institute of Biomedical Imaging and Bioengineering (NIBIB) grant P41 EB015894 and the NINDS Institutional Center Cores for Advanced Neuroimaging award P30 NS076408.

Publisher Copyright:
© 2020 John Wiley & Sons, Ltd.


  • body
  • brain
  • chemical shift displacement
  • metabolites
  • reproducibility
  • semiadiabatic LASER
  • shimming

Center for Magnetic Resonance Research (CMRR) tags

  • SMCT


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