Adult acute lymphoblastic leukemia phenotypes defined by monoclonal antibodies

R. E. Sobol, I. Royston, T. W. LeBien, J. Minowada, K. Anderson, F. R. Davey, J. Cuttner, C. Schiffer, R. R. Ellison, C. D. Bloomfield

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Pretreatment peripheral blood and/or bone marrow blasts from 90 adults with acute lymphoblastic leukemia (ALL) were analyzed as part of a prospective treatment protocol study. Specimens were tested by immunofluorescence cytofluorometry for reactivity with the following monoclonal antibodies (MoAbs): BA-1 (B cell antigen); T101, OKT11 (pan-T cell antigens [T]); 3A1 (T cell antigen); MCS-2 (myeloid antigen); J5 common ALL antigen (CALLA); BA4 (Ia antigen [Ia]); BA-2 (lymphohematopoietic antigen). Four major phenotypic groups were identified: B lineage ALL (BA-1+T-) (64%), T lineage ALL (T+BA-1-MCS-2-) (13%), unclassified ALL (BA-1-MCS-2- CALLA-T-) (9%) and myeloid antigen ALL (MCS-2+ CALLA-T-) (7%). An additional group of patients, miscellaneous ALL (7%), was comprised of cases with unusual marker profiles. In B lineage ALL, all cases tested were Ia+MCS-2-, and the vast majority were CALLA+ (84%). In T lineage ALL, 42% expressed CALLA or Ia positivity. In unclassified ALL, the predominant phenotype was Ia+BA-2+. In myeloid antigen ALL, 2 of 4 tested were 3A1+ and all cases evaluated were BA-1-. Patients with myeloid antigen ALL were older (median age, 66 years) than patients in the other groups. The T lineage ALL group had higher leukocyte counts (median WBCs, 183,000/μL) and an increased incidence of anterior mediastinal mass at presentation. All patients received identical induction therapy. In CALLA+ B lineage ALL, 30 of 46 (65%) achieved a complete remission. While the number of patients evaluated was small, 9 of 9 CALLA- B-lineage ALL and only 2 of 6 myeloid antigen ALL cases responded with a complete remission. The data suggest that these MoAbs are useful in the characterization of adult ALL.

Original languageEnglish (US)
Pages (from-to)730-735
Number of pages6
Issue number3
StatePublished - 1985

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    Sobol, R. E., Royston, I., LeBien, T. W., Minowada, J., Anderson, K., Davey, F. R., Cuttner, J., Schiffer, C., Ellison, R. R., & Bloomfield, C. D. (1985). Adult acute lymphoblastic leukemia phenotypes defined by monoclonal antibodies. Blood, 65(3), 730-735.