Adsorption of fibronectin, fibrinogen, and albumin on TiO2: time-resolved kinetics, structural changes, and competition study.

Marta Pegueroles, Chiara Tonda-Turo, Josep A. Planell, Francisco Javier Gil, Conrado Aparicio

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

An understanding of protein adsorption process is crucial for designing biomaterial surfaces. In this work, with the use of a quartz-crystal microbalance with dissipation monitoring, we researched the following: (a) the kinetics of adsorption on TiO(2) surfaces of three extensively described proteins that are relevant for metallic implant integration [i.e., albumin (BSA), fibrinogen (Fbg), and fibronectin (Fn)]; and (b) the competition of those proteins for adsorbing on TiO(2) in a two-step experiment consisted of sequentially exposing the surfaces to different monoprotein solutions. Each protein showed a different process of adsorption and properties of the adlayer-calculated using the Voigt model. The competition experiments showed that BSA displaced larger proteins such as Fn and Fbg when BSA was introduced as the second protein in the system, whereas the larger proteins laid on top of BSA forming an adsorbed protein bi-layer when those were introduced secondly in the system.

Original languageEnglish (US)
Pages (from-to)1-13
Number of pages13
JournalBiointerphases
Volume7
Issue number1-4
DOIs
StatePublished - Dec 2012

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't

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