Adhesive thermosensitive gels for local delivery of viral vectors

Jeanette M. Caronia, Daniel W. Sorensen, Hope M. Leslie, Jop van Berlo, Samira M Azarin

Research output: Contribution to journalArticle

Abstract

Local delivery of viral vectors can enhance the efficacy of therapies by selectively affecting necessary tissues and reducing the required vector dose. Pluronic F127 is a thermosensitive polymer that undergoes a solution-gelation (sol-gel) transition as temperature increases and can deliver vectors without damaging them. While pluronics can be spread over large areas, such as the surface of an organ, before gelation, they lack sufficient adhesivity to remain attached to some tissues, such as the surface of the heart or mucosal surfaces. Here, we utilized blends of pluronic F127 and polycarbophil (PCB), a mucoadhesive agent, to provide the necessary adhesivity for local delivery of viral vectors to the cardiac muscle. The effects of PCB concentration on adhesive properties, sol-gel temperature transition and cytocompatibility were evaluated. Rheological studies showed that PCB decreased the sol-gel transition temperature at concentrations >1% and increased the adhesive properties of the gel. Furthermore, these gels were able to deliver viral vectors and transduce cells in vitro and in vivo in a neonatal mouse apical resection model. These gels could be a useful platform for delivering viral vectors over the surface of organs where increased adhesivity is required.

Original languageEnglish (US)
Pages (from-to)2353-2363
Number of pages11
JournalBiotechnology and bioengineering
Volume116
Issue number9
DOIs
StatePublished - Sep 1 2019

Fingerprint

UCON 50-HB-5100
Poloxamer
Transition Temperature
Adhesives
Gels
Gelation
Superconducting transition temperature
Myocardium
Polymers
Tissue
Muscle
calcium polycarbophil
Therapeutics

Keywords

  • adhesion
  • pluronic F127
  • polycarbophil
  • solution-gelation transition
  • viral vector delivery

Cite this

Adhesive thermosensitive gels for local delivery of viral vectors. / Caronia, Jeanette M.; Sorensen, Daniel W.; Leslie, Hope M.; van Berlo, Jop; Azarin, Samira M.

In: Biotechnology and bioengineering, Vol. 116, No. 9, 01.09.2019, p. 2353-2363.

Research output: Contribution to journalArticle

Caronia, Jeanette M. ; Sorensen, Daniel W. ; Leslie, Hope M. ; van Berlo, Jop ; Azarin, Samira M. / Adhesive thermosensitive gels for local delivery of viral vectors. In: Biotechnology and bioengineering. 2019 ; Vol. 116, No. 9. pp. 2353-2363.
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