Adhesion to and invasion of cultured tick (Acarina

Ixodidae) cells by Borrelia burgdorferi (Spirochaetales: Spirochaetaceae) and maintenance of infectivity.

Timothy J Kurtti, Ulrike G Munderloh, D. E. Krueger, R. C. Johnson, T. G. Schwan

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Lyme disease spirochetes, Borrelia burgdorferi, interact with cultured tick cells in ways similar to those reported to occur in the vector Ixodes dammini Spielman, Clifford, Piesman & Corwin. Spirochete adhesion and penetration were examined using a cell line from embryos of Rhipicephalus appendiculatus Neumann that morphologically resembles tick gut cells, RAE25. Cocultivation of B. burgdorferi with these cells permitted prolonged maintenance of infectivity for hamsters. Borrelial adherence to RAE25 cells was time- and density-dependent and increased by 10-15% per h during the first 5.5 h of cocultivation when we used a concentration of 4 x 10(7) spirochetes/ml. After 6 h, > 90% of the cells bound an average of 3-5 spirochetes per cell. Low passage, hamster-infective strains of B. burgdorferi (JMNT and CD16) showed a 2-3-fold higher rate of adhesion to RAE25 cells than the highly passaged, noninfectious strain B31. Inactivation of CD16 or JMNT by heat, starvation, or treatment with puromycin reduced adherence by 40-60%, whereas pretreatment with monoclonal antibodies to the outer surface proteins had no effect. Spirochetes adhered to young I. dammini cell lines to a similar degree as they did to RAE25, whereas lines from the ticks Dermacentor variabilis (Say) (RML15) and Boophilus microplus (Canestrini) (BME26) bound 30-60% fewer spirochetes. Electron microscopy revealed epicellular borreliae associated with coated pits and vesicles before endocytosis, and intracellular spirochetes were surrounded by a host cell-derived membrane.

Original languageEnglish (US)
Pages (from-to)586-596
Number of pages11
JournalJournal of Medical Entomology
Volume30
Issue number3
DOIs
StatePublished - Jan 1 1993

Fingerprint

Spirochaetaceae
Ixodidae
Spirochaetales
Borrelia burgdorferi
Ticks
adhesion
ticks
Acari
pathogenicity
Maintenance
Ixodes
cells
Coculture Techniques
Cricetinae
Ixodes scapularis
Xamoterol
hamsters
Dermacentor
Rhipicephalus
Coated Vesicles

Cite this

@article{f1b221224cdf4cdeacb41f2ccdad0626,
title = "Adhesion to and invasion of cultured tick (Acarina: Ixodidae) cells by Borrelia burgdorferi (Spirochaetales: Spirochaetaceae) and maintenance of infectivity.",
abstract = "Lyme disease spirochetes, Borrelia burgdorferi, interact with cultured tick cells in ways similar to those reported to occur in the vector Ixodes dammini Spielman, Clifford, Piesman & Corwin. Spirochete adhesion and penetration were examined using a cell line from embryos of Rhipicephalus appendiculatus Neumann that morphologically resembles tick gut cells, RAE25. Cocultivation of B. burgdorferi with these cells permitted prolonged maintenance of infectivity for hamsters. Borrelial adherence to RAE25 cells was time- and density-dependent and increased by 10-15{\%} per h during the first 5.5 h of cocultivation when we used a concentration of 4 x 10(7) spirochetes/ml. After 6 h, > 90{\%} of the cells bound an average of 3-5 spirochetes per cell. Low passage, hamster-infective strains of B. burgdorferi (JMNT and CD16) showed a 2-3-fold higher rate of adhesion to RAE25 cells than the highly passaged, noninfectious strain B31. Inactivation of CD16 or JMNT by heat, starvation, or treatment with puromycin reduced adherence by 40-60{\%}, whereas pretreatment with monoclonal antibodies to the outer surface proteins had no effect. Spirochetes adhered to young I. dammini cell lines to a similar degree as they did to RAE25, whereas lines from the ticks Dermacentor variabilis (Say) (RML15) and Boophilus microplus (Canestrini) (BME26) bound 30-60{\%} fewer spirochetes. Electron microscopy revealed epicellular borreliae associated with coated pits and vesicles before endocytosis, and intracellular spirochetes were surrounded by a host cell-derived membrane.",
author = "Kurtti, {Timothy J} and Munderloh, {Ulrike G} and Krueger, {D. E.} and Johnson, {R. C.} and Schwan, {T. G.}",
year = "1993",
month = "1",
day = "1",
doi = "10.1093/jmedent/30.3.586",
language = "English (US)",
volume = "30",
pages = "586--596",
journal = "Journal of Medical Entomology",
issn = "0022-2585",
publisher = "Entomological Society of America",
number = "3",

}

TY - JOUR

T1 - Adhesion to and invasion of cultured tick (Acarina

T2 - Ixodidae) cells by Borrelia burgdorferi (Spirochaetales: Spirochaetaceae) and maintenance of infectivity.

AU - Kurtti, Timothy J

AU - Munderloh, Ulrike G

AU - Krueger, D. E.

AU - Johnson, R. C.

AU - Schwan, T. G.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - Lyme disease spirochetes, Borrelia burgdorferi, interact with cultured tick cells in ways similar to those reported to occur in the vector Ixodes dammini Spielman, Clifford, Piesman & Corwin. Spirochete adhesion and penetration were examined using a cell line from embryos of Rhipicephalus appendiculatus Neumann that morphologically resembles tick gut cells, RAE25. Cocultivation of B. burgdorferi with these cells permitted prolonged maintenance of infectivity for hamsters. Borrelial adherence to RAE25 cells was time- and density-dependent and increased by 10-15% per h during the first 5.5 h of cocultivation when we used a concentration of 4 x 10(7) spirochetes/ml. After 6 h, > 90% of the cells bound an average of 3-5 spirochetes per cell. Low passage, hamster-infective strains of B. burgdorferi (JMNT and CD16) showed a 2-3-fold higher rate of adhesion to RAE25 cells than the highly passaged, noninfectious strain B31. Inactivation of CD16 or JMNT by heat, starvation, or treatment with puromycin reduced adherence by 40-60%, whereas pretreatment with monoclonal antibodies to the outer surface proteins had no effect. Spirochetes adhered to young I. dammini cell lines to a similar degree as they did to RAE25, whereas lines from the ticks Dermacentor variabilis (Say) (RML15) and Boophilus microplus (Canestrini) (BME26) bound 30-60% fewer spirochetes. Electron microscopy revealed epicellular borreliae associated with coated pits and vesicles before endocytosis, and intracellular spirochetes were surrounded by a host cell-derived membrane.

AB - Lyme disease spirochetes, Borrelia burgdorferi, interact with cultured tick cells in ways similar to those reported to occur in the vector Ixodes dammini Spielman, Clifford, Piesman & Corwin. Spirochete adhesion and penetration were examined using a cell line from embryos of Rhipicephalus appendiculatus Neumann that morphologically resembles tick gut cells, RAE25. Cocultivation of B. burgdorferi with these cells permitted prolonged maintenance of infectivity for hamsters. Borrelial adherence to RAE25 cells was time- and density-dependent and increased by 10-15% per h during the first 5.5 h of cocultivation when we used a concentration of 4 x 10(7) spirochetes/ml. After 6 h, > 90% of the cells bound an average of 3-5 spirochetes per cell. Low passage, hamster-infective strains of B. burgdorferi (JMNT and CD16) showed a 2-3-fold higher rate of adhesion to RAE25 cells than the highly passaged, noninfectious strain B31. Inactivation of CD16 or JMNT by heat, starvation, or treatment with puromycin reduced adherence by 40-60%, whereas pretreatment with monoclonal antibodies to the outer surface proteins had no effect. Spirochetes adhered to young I. dammini cell lines to a similar degree as they did to RAE25, whereas lines from the ticks Dermacentor variabilis (Say) (RML15) and Boophilus microplus (Canestrini) (BME26) bound 30-60% fewer spirochetes. Electron microscopy revealed epicellular borreliae associated with coated pits and vesicles before endocytosis, and intracellular spirochetes were surrounded by a host cell-derived membrane.

UR - http://www.scopus.com/inward/record.url?scp=0027601529&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027601529&partnerID=8YFLogxK

U2 - 10.1093/jmedent/30.3.586

DO - 10.1093/jmedent/30.3.586

M3 - Article

VL - 30

SP - 586

EP - 596

JO - Journal of Medical Entomology

JF - Journal of Medical Entomology

SN - 0022-2585

IS - 3

ER -