Abstract
The adherence of Acinetobacter calcoaceticus ATCC 31012 cells to hexadecane and perfluorocarbon FC-43 was measured using the Bacterial Adherence To Hydrocarbon (BATH) assay. In batch culture the adherence of cells to both hydrophobic liquids increased sharply during the exponential growth phase and remained high for the remainder of the culture period. No correlation was found between the surface emulsan concentration and the adherence to perfluorocarbon FC-43 and hexadecane. In continuous cultures, the production of cell-free emulsan was found to be growth-associated. The adherence to both hydrophobic liquids decreased with increasing dilution rate while the amount of surface emulsan increased. Furthermore, exogenously added emulsan decreased the adherence to hydrophobic liquids. Thus, the accumulation of surface emulsan does not appear to have a beneficial effect for cell adherence to hydrophobic liquids.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 480-485 |
| Number of pages | 6 |
| Journal | Applied Microbiology and Biotechnology |
| Volume | 31 |
| Issue number | 5-6 |
| DOIs | |
| State | Published - Oct 1 1989 |