Adenovirus composition, proteolysis, and disassembly studied by in-depth qualitative and quantitative proteomics

Marco Benevento; Serena Di Palma; Joost Snijder; Crystal L. Moyer; Vijay S. Reddy; Glen R. Nemerow; Albert J.R. Heck

doi:10.1074/jbc.M113.537498

Adenovirus composition, proteolysis, and disassembly studied by in-depth qualitative and quantitative proteomics

Marco Benevento, Serena Di Palma, Joost Snijder, Crystal L. Moyer, Vijay S. Reddy, Glen R. Nemerow, Albert J.R. Heck

Research output: Contribution to journal › Article › peer-review

83 Scopus citations

Abstract

Background: Adenoviruses (AdV) are broadly employed as gene delivery vectors. Results: Copy numbers of all AdV proteins were measured, and the release of proteins upon heat stress investigated. Conclusion: The viral protease plays a distinct role in the segmented release of AdV proteins. Significance: Our characterization by mass spectrometry provides new insight in HAdV disassembly during entry into host cells.

Original language	English (US)
Pages (from-to)	11421-11430
Number of pages	10
Journal	Journal of Biological Chemistry
Volume	289
Issue number	16
DOIs	https://doi.org/10.1074/jbc.M113.537498
State	Published - Apr 18 2014
Externally published	Yes

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10.1074/jbc.M113.537498

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title = "Adenovirus composition, proteolysis, and disassembly studied by in-depth qualitative and quantitative proteomics",

abstract = "Background: Adenoviruses (AdV) are broadly employed as gene delivery vectors. Results: Copy numbers of all AdV proteins were measured, and the release of proteins upon heat stress investigated. Conclusion: The viral protease plays a distinct role in the segmented release of AdV proteins. Significance: Our characterization by mass spectrometry provides new insight in HAdV disassembly during entry into host cells.",

author = "Marco Benevento and {Di Palma}, Serena and Joost Snijder and Moyer, {Crystal L.} and Reddy, {Vijay S.} and Nemerow, {Glen R.} and Heck, {Albert J.R.}",

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doi = "10.1074/jbc.M113.537498",

language = "English (US)",

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AU - Benevento, Marco

AU - Di Palma, Serena

AU - Snijder, Joost

AU - Moyer, Crystal L.

AU - Reddy, Vijay S.

AU - Nemerow, Glen R.

AU - Heck, Albert J.R.

PY - 2014/4/18

Y1 - 2014/4/18

N2 - Background: Adenoviruses (AdV) are broadly employed as gene delivery vectors. Results: Copy numbers of all AdV proteins were measured, and the release of proteins upon heat stress investigated. Conclusion: The viral protease plays a distinct role in the segmented release of AdV proteins. Significance: Our characterization by mass spectrometry provides new insight in HAdV disassembly during entry into host cells.

AB - Background: Adenoviruses (AdV) are broadly employed as gene delivery vectors. Results: Copy numbers of all AdV proteins were measured, and the release of proteins upon heat stress investigated. Conclusion: The viral protease plays a distinct role in the segmented release of AdV proteins. Significance: Our characterization by mass spectrometry provides new insight in HAdV disassembly during entry into host cells.

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Adenovirus composition, proteolysis, and disassembly studied by in-depth qualitative and quantitative proteomics

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