Adaptation of the Müller method to allow quantitative characterization of the affinity and cross-reactivity of antibodies by competitive radioimmunoassay

Donald J. Winzor, Mark R. Bowles, Paul R. Pentel, Deric D. Schoof, Susan M. Pond

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

A quantitative expression is derived for the evaluation of antigen-antibody affinity constants from radioimmunoassays for the completely general situation in which antigen and antibody are both multivalent. The theoretical analysis is then extended to encompass quantitative characterization of the competitive inhibition observed in screening tests for cross-reactivity of antibody with structural analogs of the eliciting antigen. These procedures are illustrated with a radioimmunological study of the cross-reactivity of a desipramine-elicited monoclonal antibody with other tricyclic antidepressants. An unexpected finding to emerge from this immunochemical study is the demonstration that a single affinity constant suffices to describe the interaction of desipramine with a polyclonal antibody elicited by this univalent antigen.

Original languageEnglish (US)
Pages (from-to)995-1001
Number of pages7
JournalMolecular Immunology
Volume28
Issue number9
DOIs
StatePublished - Sep 1991

Bibliographical note

Funding Information:
*Work supported by funds from the Australian Research Council (D. J. Winzor), the Australian National Health and Medical Research Councif and P.H.S. Research Grants ES 03803 (S. M. Pond) and MH 42799 (P. R. Pentel). lIPresent address: Department of Surgery, Brigham Women’s Hospital, Boston, MA 02215, U.S.A.

Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.

Fingerprint

Dive into the research topics of 'Adaptation of the Müller method to allow quantitative characterization of the affinity and cross-reactivity of antibodies by competitive radioimmunoassay'. Together they form a unique fingerprint.

Cite this