Adaptation of FUN-1 and Calcofluor white stains to assess the ability of viable and nonviable yeast to adhere to and be internalized by cultured mammalian cells

Michelle J Henry-Stanley; Robb M. Garni; Carol L Wells

doi:10.1016/j.mimet.2004.07.001

Adaptation of FUN-1 and Calcofluor white stains to assess the ability of viable and nonviable yeast to adhere to and be internalized by cultured mammalian cells

Michelle J Henry-Stanley
, Robb M. Garni
, Carol L Wells

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

The FUN-1 and Calcofluor white stains can be used in concert to assess the ability of viable and nonviable yeast to adhere to, and be internalized by, host mammalian cells in vitro. With this method, only extracellular yeast stain with Calcofluor, dead yeast cells have diffuse cytoplasmic yellow-green fluorescence, and live yeast have cytoplasmic orange-red or yellow-orange fluorescent intravacuolar structures.

Original language	English (US)
Pages (from-to)	289-292
Number of pages	4
Journal	Journal of Microbiological Methods
Volume	59
Issue number	2
DOIs	https://doi.org/10.1016/j.mimet.2004.07.001
State	Published - Nov 2004

Bibliographical note

Funding Information:
This work was supported in part by Public Health Service grant GM 059221 from the National Institutes of Health, USA.

Keywords

Calcofluor white
FUN-1
Intracellular
Viability
Yeast

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10.1016/j.mimet.2004.07.001

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title = "Adaptation of FUN-1 and Calcofluor white stains to assess the ability of viable and nonviable yeast to adhere to and be internalized by cultured mammalian cells",

abstract = "The FUN-1 and Calcofluor white stains can be used in concert to assess the ability of viable and nonviable yeast to adhere to, and be internalized by, host mammalian cells in vitro. With this method, only extracellular yeast stain with Calcofluor, dead yeast cells have diffuse cytoplasmic yellow-green fluorescence, and live yeast have cytoplasmic orange-red or yellow-orange fluorescent intravacuolar structures.",

keywords = "Calcofluor white, FUN-1, Intracellular, Viability, Yeast",

author = "Henry-Stanley, \{Michelle J\} and Garni, \{Robb M.\} and Wells, \{Carol L\}",

note = "Funding Information: This work was supported in part by Public Health Service grant GM 059221 from the National Institutes of Health, USA.",

year = "2004",

month = nov,

doi = "10.1016/j.mimet.2004.07.001",

language = "English (US)",

volume = "59",

pages = "289--292",

journal = "Journal of Microbiological Methods",

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T1 - Adaptation of FUN-1 and Calcofluor white stains to assess the ability of viable and nonviable yeast to adhere to and be internalized by cultured mammalian cells

AU - Henry-Stanley, Michelle J

AU - Garni, Robb M.

AU - Wells, Carol L

N1 - Funding Information: This work was supported in part by Public Health Service grant GM 059221 from the National Institutes of Health, USA.

PY - 2004/11

Y1 - 2004/11

N2 - The FUN-1 and Calcofluor white stains can be used in concert to assess the ability of viable and nonviable yeast to adhere to, and be internalized by, host mammalian cells in vitro. With this method, only extracellular yeast stain with Calcofluor, dead yeast cells have diffuse cytoplasmic yellow-green fluorescence, and live yeast have cytoplasmic orange-red or yellow-orange fluorescent intravacuolar structures.

AB - The FUN-1 and Calcofluor white stains can be used in concert to assess the ability of viable and nonviable yeast to adhere to, and be internalized by, host mammalian cells in vitro. With this method, only extracellular yeast stain with Calcofluor, dead yeast cells have diffuse cytoplasmic yellow-green fluorescence, and live yeast have cytoplasmic orange-red or yellow-orange fluorescent intravacuolar structures.

KW - Calcofluor white

KW - FUN-1

KW - Intracellular

KW - Viability

KW - Yeast

UR - https://www.scopus.com/pages/publications/5344227637

UR - https://www.scopus.com/pages/publications/5344227637#tab=citedBy

U2 - 10.1016/j.mimet.2004.07.001

DO - 10.1016/j.mimet.2004.07.001

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AN - SCOPUS:5344227637

SN - 0167-7012

VL - 59

SP - 289

EP - 292

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 2

ER -

Adaptation of FUN-1 and Calcofluor white stains to assess the ability of viable and nonviable yeast to adhere to and be internalized by cultured mammalian cells

Abstract

Bibliographical note

Keywords

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