Adaptation of FUN-1 and Calcofluor white stains to assess the ability of viable and nonviable yeast to adhere to and be internalized by cultured mammalian cells

Michelle J Henry-Stanley, Robb M. Garni, Carol L Wells

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

The FUN-1 and Calcofluor white stains can be used in concert to assess the ability of viable and nonviable yeast to adhere to, and be internalized by, host mammalian cells in vitro. With this method, only extracellular yeast stain with Calcofluor, dead yeast cells have diffuse cytoplasmic yellow-green fluorescence, and live yeast have cytoplasmic orange-red or yellow-orange fluorescent intravacuolar structures.

Original languageEnglish (US)
Pages (from-to)289-292
Number of pages4
JournalJournal of Microbiological Methods
Volume59
Issue number2
DOIs
StatePublished - Nov 2004

Bibliographical note

Funding Information:
This work was supported in part by Public Health Service grant GM 059221 from the National Institutes of Health, USA.

Keywords

  • Calcofluor white
  • FUN-1
  • Intracellular
  • Viability
  • Yeast

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