Acute and chronic opiate action in NG108-15 hybrid cells cultured in serum-free medium

M. T. Griffin, P. Y. Law, H. H. Loh

Research output: Contribution to journalArticle

Abstract

We have demonstrated that NG108-15 hybrid cells can poliferate for several weeks in the absence of serum, and therefore serum lipids, if synthetic medium is supplemented with specific hormones, trace elements and nutrients. The culturing of NG108-15 hybrid cells under serum free growth conditions did not alter opiate acute or chronic regulation of adenylate cyclase activity. In addition, we determined that the affinity and numbers of opiate receptors as well as the magnitude and IC50 of etorphine inhibition of adenylate cyclase was not altered when cells were grown in DM. Growth in serum free, chemically defined medium forces the cell to rely entirely on endogenous lipids which will eventually lead to an alteration of acyl group composition in membrane phosphatides. Therefore, serum lipids or the alteration of acyl group composition must not be involved in the expression of the opiate dependent responses, acute and chronic, observed in this cell line.

Original languageEnglish (US)
Pages (from-to)561-564
Number of pages4
JournalProceedings of the Western Pharmacology Society
VolumeVOL. 27
StatePublished - Jan 1 1984

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