Activity-based protein profiling methods to study bacteria

The power of small-molecule electrophiles

Shabnam Sharifzadeh, Joshua D. Shirley, Erin E Carlson

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

ABPP methods have been utilized for the last two decades as a means to investigate complex proteomes in all three domains of life. Extensive use in eukaryotes has provided a more fundamental understanding of the biological processes involved in numerous diseases and has driven drug discovery and treatment campaigns. However, the use of ABPP in prokaryotes has been less common, although it has gained more attention over the last decade. The urgent need for understanding bacteriophysiology and bacterial pathogenicity at a foundational level has never been more apparent, as the rise in antibiotic resistance has resulted in the inadequate and ineffective treatment of infections. This is not only a result of resistance to clinically used antibiotics, but also a lack of new drugs and equally as important, new drug targets. ABPP provides a means for which new, clinically relevant drug targets may be identified through gaining insight into biological processes. In this chapter, we place particular focus on the discussion of ABPP strategies that have been applied to study different classes of bacterial enzymes.

Original languageEnglish (US)
Title of host publicationCurrent Topics in Microbiology and Immunology
PublisherSpringer- Verlag
Pages23-48
Number of pages26
DOIs
StatePublished - Jan 1 2019

Publication series

NameCurrent Topics in Microbiology and Immunology
Volume420
ISSN (Print)0070-217X
ISSN (Electronic)2196-9965

Fingerprint

Biological Phenomena
Bacteria
Pharmaceutical Preparations
Proteins
Drug Discovery
Proteome
Microbial Drug Resistance
Eukaryota
Virulence
Anti-Bacterial Agents
Enzymes
Infection

PubMed: MeSH publication types

  • Journal Article
  • Review

Cite this

Sharifzadeh, S., Shirley, J. D., & Carlson, E. E. (2019). Activity-based protein profiling methods to study bacteria: The power of small-molecule electrophiles. In Current Topics in Microbiology and Immunology (pp. 23-48). (Current Topics in Microbiology and Immunology; Vol. 420). Springer- Verlag. https://doi.org/10.1007/82_2018_135

Activity-based protein profiling methods to study bacteria : The power of small-molecule electrophiles. / Sharifzadeh, Shabnam; Shirley, Joshua D.; Carlson, Erin E.

Current Topics in Microbiology and Immunology. Springer- Verlag, 2019. p. 23-48 (Current Topics in Microbiology and Immunology; Vol. 420).

Research output: Chapter in Book/Report/Conference proceedingChapter

Sharifzadeh, S, Shirley, JD & Carlson, EE 2019, Activity-based protein profiling methods to study bacteria: The power of small-molecule electrophiles. in Current Topics in Microbiology and Immunology. Current Topics in Microbiology and Immunology, vol. 420, Springer- Verlag, pp. 23-48. https://doi.org/10.1007/82_2018_135
Sharifzadeh S, Shirley JD, Carlson EE. Activity-based protein profiling methods to study bacteria: The power of small-molecule electrophiles. In Current Topics in Microbiology and Immunology. Springer- Verlag. 2019. p. 23-48. (Current Topics in Microbiology and Immunology). https://doi.org/10.1007/82_2018_135
Sharifzadeh, Shabnam ; Shirley, Joshua D. ; Carlson, Erin E. / Activity-based protein profiling methods to study bacteria : The power of small-molecule electrophiles. Current Topics in Microbiology and Immunology. Springer- Verlag, 2019. pp. 23-48 (Current Topics in Microbiology and Immunology).
@inbook{1605db0a56504c179096143bc309d946,
title = "Activity-based protein profiling methods to study bacteria: The power of small-molecule electrophiles",
abstract = "ABPP methods have been utilized for the last two decades as a means to investigate complex proteomes in all three domains of life. Extensive use in eukaryotes has provided a more fundamental understanding of the biological processes involved in numerous diseases and has driven drug discovery and treatment campaigns. However, the use of ABPP in prokaryotes has been less common, although it has gained more attention over the last decade. The urgent need for understanding bacteriophysiology and bacterial pathogenicity at a foundational level has never been more apparent, as the rise in antibiotic resistance has resulted in the inadequate and ineffective treatment of infections. This is not only a result of resistance to clinically used antibiotics, but also a lack of new drugs and equally as important, new drug targets. ABPP provides a means for which new, clinically relevant drug targets may be identified through gaining insight into biological processes. In this chapter, we place particular focus on the discussion of ABPP strategies that have been applied to study different classes of bacterial enzymes.",
author = "Shabnam Sharifzadeh and Shirley, {Joshua D.} and Carlson, {Erin E}",
year = "2019",
month = "1",
day = "1",
doi = "10.1007/82_2018_135",
language = "English (US)",
series = "Current Topics in Microbiology and Immunology",
publisher = "Springer- Verlag",
pages = "23--48",
booktitle = "Current Topics in Microbiology and Immunology",

}

TY - CHAP

T1 - Activity-based protein profiling methods to study bacteria

T2 - The power of small-molecule electrophiles

AU - Sharifzadeh, Shabnam

AU - Shirley, Joshua D.

AU - Carlson, Erin E

PY - 2019/1/1

Y1 - 2019/1/1

N2 - ABPP methods have been utilized for the last two decades as a means to investigate complex proteomes in all three domains of life. Extensive use in eukaryotes has provided a more fundamental understanding of the biological processes involved in numerous diseases and has driven drug discovery and treatment campaigns. However, the use of ABPP in prokaryotes has been less common, although it has gained more attention over the last decade. The urgent need for understanding bacteriophysiology and bacterial pathogenicity at a foundational level has never been more apparent, as the rise in antibiotic resistance has resulted in the inadequate and ineffective treatment of infections. This is not only a result of resistance to clinically used antibiotics, but also a lack of new drugs and equally as important, new drug targets. ABPP provides a means for which new, clinically relevant drug targets may be identified through gaining insight into biological processes. In this chapter, we place particular focus on the discussion of ABPP strategies that have been applied to study different classes of bacterial enzymes.

AB - ABPP methods have been utilized for the last two decades as a means to investigate complex proteomes in all three domains of life. Extensive use in eukaryotes has provided a more fundamental understanding of the biological processes involved in numerous diseases and has driven drug discovery and treatment campaigns. However, the use of ABPP in prokaryotes has been less common, although it has gained more attention over the last decade. The urgent need for understanding bacteriophysiology and bacterial pathogenicity at a foundational level has never been more apparent, as the rise in antibiotic resistance has resulted in the inadequate and ineffective treatment of infections. This is not only a result of resistance to clinically used antibiotics, but also a lack of new drugs and equally as important, new drug targets. ABPP provides a means for which new, clinically relevant drug targets may be identified through gaining insight into biological processes. In this chapter, we place particular focus on the discussion of ABPP strategies that have been applied to study different classes of bacterial enzymes.

UR - http://www.scopus.com/inward/record.url?scp=85060554574&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85060554574&partnerID=8YFLogxK

U2 - 10.1007/82_2018_135

DO - 10.1007/82_2018_135

M3 - Chapter

T3 - Current Topics in Microbiology and Immunology

SP - 23

EP - 48

BT - Current Topics in Microbiology and Immunology

PB - Springer- Verlag

ER -