Active repression of organizer genes by C-terminal domain of PV.1

Yoo Seok Hwang, Hyun Shik Lee, Dong Hyun Roh, Sang Wook Cha, Sung Young Lee, Jeong Jae Seo, Jaebong Kim, Mae Ja Park

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

PV.1, a homeotic protein, ventralizes dorsal mesoderm and inhibits neuralization by mediating BMP-4 signaling in Xenopus embryo. In our previous report antimorphic PV.1 causes a secondary axis by inducing the ectopic organizer. We analyzed the structure of this transcription factor through domain level assessment. In a phenotype-inducing test, half of the N-terminus at the N-terminal side was unessential for inducing ventralization of embryos. We examined the transacting activity of several regions of PV.1 utilizing GAL4 hybrid system. The C-terminal region/GAL4DBD (DNA binding domain) exhibited strong repressive activity on a reporter gene (operator/promoter/reporter; Gal4-TK-luc) as much as the whole polypeptide/GAL4DBD, whereas the N-terminal region/GAL4DBD showed only modest repression. The results suggest that PV.1 functions as a transcriptional repressor and this repressive activity is localized mostly to the C-terminal region. Additional characterizations of N- and C-terminus with respect to the effects on the expression of other genes are described.

Original languageEnglish (US)
Pages (from-to)79-86
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume308
Issue number1
DOIs
StatePublished - Aug 15 2003

Keywords

  • Active repression
  • BMP-4
  • Dorsoventral axis
  • GAL4
  • Homeodomain
  • PV.1
  • Proline-rich
  • Repression domain
  • Transcriptional regulation
  • Ventralization

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