Abstract
PV.1, a homeotic protein, ventralizes dorsal mesoderm and inhibits neuralization by mediating BMP-4 signaling in Xenopus embryo. In our previous report antimorphic PV.1 causes a secondary axis by inducing the ectopic organizer. We analyzed the structure of this transcription factor through domain level assessment. In a phenotype-inducing test, half of the N-terminus at the N-terminal side was unessential for inducing ventralization of embryos. We examined the transacting activity of several regions of PV.1 utilizing GAL4 hybrid system. The C-terminal region/GAL4DBD (DNA binding domain) exhibited strong repressive activity on a reporter gene (operator/promoter/reporter; Gal4-TK-luc) as much as the whole polypeptide/GAL4DBD, whereas the N-terminal region/GAL4DBD showed only modest repression. The results suggest that PV.1 functions as a transcriptional repressor and this repressive activity is localized mostly to the C-terminal region. Additional characterizations of N- and C-terminus with respect to the effects on the expression of other genes are described.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 79-86 |
| Number of pages | 8 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 308 |
| Issue number | 1 |
| DOIs | |
| State | Published - Aug 15 2003 |
Bibliographical note
Funding Information:We thank Dr. Ding for pSK-2066, the noggin promoter, and Dr. K.T. Ault, for PV.1 cDNA. We also thank Dr. Hueng-Sik Choi for sharing GAL4-TK-Luc and pCMX-GAL4. This work was supported by Grant No. R05-2001-000-00473-0 from the Basic Research Program of the Korea Science and Engineering Foundation.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
Keywords
- Active repression
- BMP-4
- Dorsoventral axis
- GAL4
- Homeodomain
- PV.1
- Proline-rich
- Repression domain
- Transcriptional regulation
- Ventralization