Activation of the unfolded protein response downregulates cardiac ion channels in human induced pluripotent stem cell-derived cardiomyocytes

Man Liu, Guangbin Shi, Anyu Zhou, Cassady E. Rupert, Kareen L.K. Coulombe, Samuel C. Dudley

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Rationale: Heart failure is characterized by electrical remodeling that contributes to arrhythmic risk. The unfolded protein response (UPR) is active in heart failure and can decrease protein levels by increasing mRNA decay, accelerating protein degradation, and inhibiting protein translation. Objective: Therefore, we investigated whether the UPR downregulated cardiac ion channels that may contribute to arrhythmogenic electrical remodeling. Methods: Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) were used to study cardiac ion channels. Action potentials (APs) and ion channel currents were measured by patch clamp recording. The mRNA and protein levels of channels and the UPR effectors were determined by quantitative RT-PCR and Western blotting. Tunicamycin (TM, 50 ng/mL and 5 μg/mL), GSK2606414 (GSK, 300 nmol/L), and 4μ8C (5 μmol/L) were utilized to activate the UPR, inhibit protein kinase-like ER kinase (PERK) and inositol-requiring protein-1 (IRE1), respectively. Results: TM-induced activation of the UPR caused significant prolongation of the AP duration (APD) and a reduction of the maximum upstroke velocity (dV/dt max ) of the AP phase 0 in both acute (20–24 h) and chronic treatment (6 days). These changes were explained by reductions in the sodium, L-type calcium, the transient outward and rapidly/slowly activating delayed rectifier potassium currents. Na v 1.5, Ca v 1.2, K v 4.3, and K v LQT1 channels showed concomitant reductions in mRNA and protein levels under activated UPR. Inhibition of PERK or IRE1 shortened the APD and reinstated dV/dt max . The PERK branch regulated Na v 1.5, K v 4.3, hERG, and K v LQT1. The IRE1 branch regulated Na v 1.5, hERG, K v LQT1, and Ca v 1.2. Conclusions: Activated UPR downregulates all major cardiac ion currents and results in electrical remodeling in hiPSC-CMs. Both PERK and IRE1 branches downregulate Na v 1.5, hERG, and K v LQT1. The PERK branch specifically downregulates K v 4.3, while the IRE1 branch downregulates Ca v 1.2. Therefore, the UPR contributed to electrical remodeling, and targeting the UPR might be anti-arrhythmic.

Original languageEnglish (US)
Pages (from-to)62-71
Number of pages10
JournalJournal of Molecular and Cellular Cardiology
Volume117
DOIs
StatePublished - Apr 2018

Bibliographical note

Funding Information:
This work was supported by Rhode Island Foundation grant 20154145 (ML) and R01 HL104025 (SCD).

Publisher Copyright:
© 2018 Elsevier Ltd

Keywords

  • Heart failure
  • IRE1
  • PERK
  • hiPSC-derived cardiomyocytes

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