Activation of the Lck tyrosine-protein kinase by the binding of the Tip protein of herpesvirus saimiri in the absence of regulatory tyrosine phosphorylation

David A. Hartley, Tamara R. Hurley, James S. Hardwick, Troy C. Lund, Peter G. Medveczky, Bartholomew M. Sefton

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

The Tip protein of herpesvirus saimiri 484 binds to the Lek tyrosine- protein kinase at two sites and activates it dramatically. Lck has been shown previously to be activated by either phosphorylation of Tyr394 or dephosphorylation of Tyr505. We examined here whether a change in the phosphorylation of either site was required for the activation of Lck by Tip. Remarkably, mutation of both regulatory sites of tyrosine phosphorylation did not prevent activation of Lck by Tip either in vivo or in a cell free in vitro system. Tip therefore appears to be able to activate Lck through an induced conformational change that does not necessarily involve altered phosphorylation of the kinase. Tip may represent the prototype of a novel type of regulator of tyrosine-protein kinases.

Original languageEnglish (US)
Pages (from-to)20056-20059
Number of pages4
JournalJournal of Biological Chemistry
Volume274
Issue number29
DOIs
StatePublished - Jul 16 1999

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