Activation of spinal microglia in a murine model of peripheral inflammation-induced, long-lasting contralateral allodynia

Kristin L. Schreiber, Al J Beitz, George L Wilcox

Research output: Contribution to journalArticlepeer-review

52 Scopus citations

Abstract

Increased sensitivity contralateral to an injury has been described in humans and in various models of neuropathic pain in rats. The mechanism underlying contralateral hypersensitivity is as yet unclear, although previous studies have implicated involvement of both spinal neurons and glia. We describe the development of a temporally delayed, robust and long-lasting contralateral allodynia in mice after hindpaw injection with 4% carrageenan. Both ipsilateral and contralateral allodynia could be inhibited temporarily by intrathecally administered morphine, clonidine, or neostigmine. The delayed development of contralateral allodynia correlated with an increase in OX-42, but not GFAP immunoreactivity in the contralateral dorsal horn. Furthermore, intrathecal treatment with minocycline inhibited the development of contralateral allodynia, suggesting that microglial activation plays a key role in contralateralization, and may be a potential target for clinical intervention after injury or inflammation has occurred, to eliminate the subsequent development of extraterritorial pain.

Original languageEnglish (US)
Pages (from-to)63-67
Number of pages5
JournalNeuroscience Letters
Volume440
Issue number1
DOIs
StatePublished - Jul 25 2008

Keywords

  • Carrageenan
  • Contralateral allodynia
  • Inflammatory pain
  • Microglia
  • Mouse
  • Spinal cord

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