Activation of NF-κB/Rel in angelan-stimulated macrophages

Young J Jeon, Sang Bae Han, Kyung Seop Ahn, Hwan Mook Kim

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

In our previous studies we showed that the primary target cell of angelan, a polysaccharide purified from Angelica gigas Nakai, is a macrophage (). In the present study we examined the effect of angelan on iNOS, IL-1β, and TNF-α transcription in mouse macrophage line RAW 264.7. We show that angelan produces a marked induction of iNOS, IL-1β, and TNF-α transcription by RAW 264.7 cells. Since these gene transcriptions have been recently shown to be under the control of NF-κB/Rel family of transcription factors, we assessed the effect of angelan on NF-κB/Rel using a electrophoretic mobility shift assay. Treatment of RAW 264.7 cells with angelan produced strong induction of NF-κB/Rel binding. Treatment of RAW 264.7 cells with angelan slightly induced AP-1 binding activity, whereas Oct binding was not affected by angelan. Angelan stimulated macrophages to activate NF-κB/Rel, whereas neither B-cells nor T-cells were affected by the angelan. In conclusion, we demonstrate that the stimulation effect of angelan on macrophage is mediated by specific activation of NF-κB/Rel. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalImmunopharmacology
Volume43
Issue number1
DOIs
StatePublished - Jun 1 1999

Keywords

  • Angelan
  • Angelica gigas Nakai
  • IL-1β
  • Immunostimulation
  • Macrophages
  • TNF-α
  • iNOS

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