Abstract
In our previous studies we showed that the primary target cell of angelan, a polysaccharide purified from Angelica gigas Nakai, is a macrophage (). In the present study we examined the effect of angelan on iNOS, IL-1β, and TNF-α transcription in mouse macrophage line RAW 264.7. We show that angelan produces a marked induction of iNOS, IL-1β, and TNF-α transcription by RAW 264.7 cells. Since these gene transcriptions have been recently shown to be under the control of NF-κB/Rel family of transcription factors, we assessed the effect of angelan on NF-κB/Rel using a electrophoretic mobility shift assay. Treatment of RAW 264.7 cells with angelan produced strong induction of NF-κB/Rel binding. Treatment of RAW 264.7 cells with angelan slightly induced AP-1 binding activity, whereas Oct binding was not affected by angelan. Angelan stimulated macrophages to activate NF-κB/Rel, whereas neither B-cells nor T-cells were affected by the angelan. In conclusion, we demonstrate that the stimulation effect of angelan on macrophage is mediated by specific activation of NF-κB/Rel. Copyright (C) 1999 Elsevier Science B.V.
Original language | English (US) |
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Pages (from-to) | 1-9 |
Number of pages | 9 |
Journal | Immunopharmacology |
Volume | 43 |
Issue number | 1 |
DOIs | |
State | Published - Jun 1999 |
Externally published | Yes |
Bibliographical note
Copyright:Copyright 2007 Elsevier B.V., All rights reserved.
Keywords
- Angelan
- Angelica gigas Nakai
- IL-1β
- Immunostimulation
- Macrophages
- TNF-α
- iNOS