Abstract
Recent studies indicate that the mycotoxin and putative carcinogen fumonisin B1 stimulates mitogenesis in Swiss 3T3 fibroblasts. Because mitogen-activated protein kinase (MAPK) is a key enzyme in the signal transduction pathways activated by many mitogens, we hypothesized that fumonisin B1 might modulate MAPK activity. Consistent with other reports, we observed that fumonisin B1 is synergistic with insulin for mitogenesis in Swiss 3T3 cells. We also observed that: (1) fumonisin B1 stimulates rapid, transient activation of MAPK; and (2) the dose-response curve for fumonisin B1-stimulated activation of MAPK is similar to the dose-response curve for the mitogenic effects of fumonisin B1. In contrast to fumonisin B1 insulin stimulates minimal activation of MAPK in Swiss 3T3 cells. The observation that fumonisin B1 and insulin differ with respect to the modulation of MAPK activity suggests a possible mechanism for their synergistic stimulation of mitogenesis in Swiss 3T3 cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 622-627 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 227 |
| Issue number | 2 |
| DOIs | |
| State | Published - Oct 14 1996 |
Bibliographical note
Funding Information:We would like to thank Dr. Marsha Rich Rosner of the Ben May Institute, University of Chicago, and the members of the Rosner laboratory for their generosity and assistance in helping to start these studies. This work was supported in part by American Cancer Society Institutional Research Grant IN-13-35-17 (E.V.W.), the Grant-in-Aid Program of the Office of the Vice President for Research at the University of Minnesota, (E.V.W.) and Grant 93-37201-9561 awarded by NRICGP/USDA (W.T.S.).