Activation of dihaloalkanes by glutathione conjugation and formation of DNA adducts

F. P. Guengerich, L. A. Peterson, J. L. Cmarik, N. Koga, P. B. Inskeep

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Ethylene dibromide (1,2-dibromoethane, EDB) can be activated to electrophilic species by either oxidative metabolism or conjugation with glutathione. Although conjugation is generally a route of detoxication, in this case it leads to genetic damage. The major DNA adduct has been identified as S-[2-(N7-guanyl)ethyl]glutathione, which is believed to arise via half-mustard and episulfonium ion intermediates. The adduct has a half-life of about 70 to 100 hr and does not appear to migrate to other DNA sites. Glutathione-dependent DNA damage by EDB was also demonstrated in human hepatocyte preparations. The possible relevance of this DNA adduct to genetic damage is discussed.

Original languageEnglish (US)
Pages (from-to)15-18
Number of pages4
JournalEnvironmental health perspectives
Volume76
DOIs
StatePublished - 1987

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