Activation of antigen-specific T cells by artificial cell constructs having immobilized multimeric peptide-class I complexes and recombinant B7-Fc proteins

Ee Loon Tham, Paige L. Jensen, Matthew F. Mescher

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

T cell activation results from the engagement of multiple receptors on T cells by their respective ligands on antigen presenting cells. Studies using artificial cell surface constructs have demonstrated that effective T cell response requires that antigen be presented on a solid surface with dimensions that approximate those of an intact cell. In this report, we describe the cloning and expression of recombinant B7-1-Fc and B7-2-Fc proteins and their incorporation onto 5-μm latex microspheres along with renatured peptide-MHC. These microspheres provide a simple and effective method for the in vitro or in vivo stimulation of antigen-specific T cells under precisely controlled antigen and costimulation conditions.

Original languageEnglish (US)
Pages (from-to)111-119
Number of pages9
JournalJournal of Immunological Methods
Volume249
Issue number1-2
DOIs
StatePublished - Mar 1 2001

Keywords

  • Artificial cell surface constructs
  • Costimulation
  • Lymphocyte activation

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