Activation-dependent changes in soluble fibronectin binding and expression of β1 integrin activation epitopes in T cells: Relationship to T cell adhesion and migration

Melody L. Woods, Carlos Cabañas, Yoji Shimizu

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

The relationship between activation-dependent changes in β1 integrin conformation, T cell adhesion to immobilized fibronectin, and T cell migration in vitro was analyzed in this study. Stimulation of Jurkat T cells and peripheral T cells with Mn2+, the activating β1 integrin-specific monoclonal antibody (mAb) TS2/16, CD2, or CD28 stimulation led to increased adhesion, soluble fibronectin (FN) binding and expression of the activation epitope defined by the β1 integrin mAb HUTS-21. Phorbol 12-myristate 13-acetate treatment increased adhesion, but not soluble FN binding or HUTS-21 epitope expression. In peripheral T cells, CD3 or CD7 stimulation also led to increased adhesion, soluble FN binding and HUTS-21 epitope expression. Soluble FN blocked peripheral T cell adhesion induced by Mn2+ or TS2/16, but had no effect on adhesion induced by the other integrin-activating signals. In contrast, migration induced by TS2/16, CD2, CD3, CD7 or CD28 stimulation was blocked by excess soluble FN. Phosphoinositide 3-OH kinase (PI 3-K) inhibitors blocked receptor-mediated increases in cell adhesion, but not soluble FN binding or HUTS-21 expression. Migration was similarly unaffected by PI 3-K inhibitors, with the exception of CD7- and CD28-induced migration, which was specifically blocked by LY294,002. These results suggest that activation-dependent changes in β1 integrin conformation are PI 3-K-independent and are involved in T cell migration but not adhesion.

Original languageEnglish (US)
Pages (from-to)38-49
Number of pages12
JournalEuropean Journal of Immunology
Volume30
Issue number1
DOIs
StatePublished - 2000

Keywords

  • Adhesion molecule
  • Cellular activation
  • T lymphocyte

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