Activation and expression of ERK, JNK, and p38 MAP-kinases in isolated islets of Langerhans: Implications for cultured islet survival

Steven Paraskevas, Reid Aikin, Dusica Maysinger, Jonathan R.T. Lakey, Thomas J. Cavanagh, Bernhard Hering, Rennian Wang, Lawrence Rosenberg

Research output: Contribution to journalArticlepeer-review

75 Scopus citations

Abstract

Isolation and purification of islet cells exposes them to ischemic, osmotic and mechanical stresses. The objective of this study was to determine the roles of the MAP-kinases in islets immediately following isolation. During the first 48 h, activity of JNK1 and JNK2 declined markedly. Activity of p38 increased steadily with time in culture while extracellular signal regulated kinase (ERK) activity declined dramatically within 24 h post-isolation. High p38 activation relative to ERK activation immediately following isolation correlated with a decrease in islet survival after 36 h in culture. Absence and/or transiency of ERK signaling in conjunction with sustained activation of p38 pathway could be an important regulator of cell death in islets during and following their isolation by commonly employed procedures. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish (US)
Pages (from-to)203-208
Number of pages6
JournalFEBS Letters
Volume455
Issue number3
DOIs
StatePublished - Jul 23 1999

Bibliographical note

Funding Information:
The work was supported (D.M. and L.R.) by the Canadian Diabetes Association. S.P. was supported by a fellowship from the Canadian Diabetes Association. J.R.T.L. was supported by a post-doctoral fellowship from the Alberta Heritage Foundation for Medical Research

Keywords

  • Apoptosis
  • Extracellular signal regulated kinase
  • Islets of Langerhans
  • JNK
  • Mitogen activated protein kinase
  • p38

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