Activation and expression of ERK, JNK, and p38 MAP-kinases in isolated islets of Langerhans: Implications for cultured islet survival

Steven Paraskevas; Reid Aikin; Dusica Maysinger; Jonathan R.T. Lakey; Thomas J. Cavanagh; Bernhard Hering; Rennian Wang; Lawrence Rosenberg

doi:10.1016/S0014-5793(99)00882-0

Activation and expression of ERK, JNK, and p38 MAP-kinases in isolated islets of Langerhans: Implications for cultured islet survival

Steven Paraskevas, Reid Aikin, Dusica Maysinger, Jonathan R.T. Lakey, Thomas J. Cavanagh, Bernhard Hering, Rennian Wang, Lawrence Rosenberg

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Abstract

Isolation and purification of islet cells exposes them to ischemic, osmotic and mechanical stresses. The objective of this study was to determine the roles of the MAP-kinases in islets immediately following isolation. During the first 48 h, activity of JNK1 and JNK2 declined markedly. Activity of p38 increased steadily with time in culture while extracellular signal regulated kinase (ERK) activity declined dramatically within 24 h post-isolation. High p38 activation relative to ERK activation immediately following isolation correlated with a decrease in islet survival after 36 h in culture. Absence and/or transiency of ERK signaling in conjunction with sustained activation of p38 pathway could be an important regulator of cell death in islets during and following their isolation by commonly employed procedures. Copyright (C) 1999 Federation of European Biochemical Societies.

Original language	English (US)
Pages (from-to)	203-208
Number of pages	6
Journal	FEBS Letters
Volume	455
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(99)00882-0
State	Published - Jul 23 1999

Bibliographical note

Funding Information:
The work was supported (D.M. and L.R.) by the Canadian Diabetes Association. S.P. was supported by a fellowship from the Canadian Diabetes Association. J.R.T.L. was supported by a post-doctoral fellowship from the Alberta Heritage Foundation for Medical Research

Keywords

Apoptosis
Extracellular signal regulated kinase
Islets of Langerhans
JNK
Mitogen activated protein kinase
p38

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10.1016/S0014-5793(99)00882-0

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title = "Activation and expression of ERK, JNK, and p38 MAP-kinases in isolated islets of Langerhans: Implications for cultured islet survival",

abstract = "Isolation and purification of islet cells exposes them to ischemic, osmotic and mechanical stresses. The objective of this study was to determine the roles of the MAP-kinases in islets immediately following isolation. During the first 48 h, activity of JNK1 and JNK2 declined markedly. Activity of p38 increased steadily with time in culture while extracellular signal regulated kinase (ERK) activity declined dramatically within 24 h post-isolation. High p38 activation relative to ERK activation immediately following isolation correlated with a decrease in islet survival after 36 h in culture. Absence and/or transiency of ERK signaling in conjunction with sustained activation of p38 pathway could be an important regulator of cell death in islets during and following their isolation by commonly employed procedures. Copyright (C) 1999 Federation of European Biochemical Societies.",

keywords = "Apoptosis, Extracellular signal regulated kinase, Islets of Langerhans, JNK, Mitogen activated protein kinase, p38",

author = "Steven Paraskevas and Reid Aikin and Dusica Maysinger and Lakey, {Jonathan R.T.} and Cavanagh, {Thomas J.} and Bernhard Hering and Rennian Wang and Lawrence Rosenberg",

note = "Funding Information: The work was supported (D.M. and L.R.) by the Canadian Diabetes Association. S.P. was supported by a fellowship from the Canadian Diabetes Association. J.R.T.L. was supported by a post-doctoral fellowship from the Alberta Heritage Foundation for Medical Research",

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doi = "10.1016/S0014-5793(99)00882-0",

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AU - Aikin, Reid

AU - Maysinger, Dusica

AU - Lakey, Jonathan R.T.

AU - Cavanagh, Thomas J.

AU - Hering, Bernhard

AU - Wang, Rennian

AU - Rosenberg, Lawrence

N1 - Funding Information: The work was supported (D.M. and L.R.) by the Canadian Diabetes Association. S.P. was supported by a fellowship from the Canadian Diabetes Association. J.R.T.L. was supported by a post-doctoral fellowship from the Alberta Heritage Foundation for Medical Research

PY - 1999/7/23

Y1 - 1999/7/23

N2 - Isolation and purification of islet cells exposes them to ischemic, osmotic and mechanical stresses. The objective of this study was to determine the roles of the MAP-kinases in islets immediately following isolation. During the first 48 h, activity of JNK1 and JNK2 declined markedly. Activity of p38 increased steadily with time in culture while extracellular signal regulated kinase (ERK) activity declined dramatically within 24 h post-isolation. High p38 activation relative to ERK activation immediately following isolation correlated with a decrease in islet survival after 36 h in culture. Absence and/or transiency of ERK signaling in conjunction with sustained activation of p38 pathway could be an important regulator of cell death in islets during and following their isolation by commonly employed procedures. Copyright (C) 1999 Federation of European Biochemical Societies.

AB - Isolation and purification of islet cells exposes them to ischemic, osmotic and mechanical stresses. The objective of this study was to determine the roles of the MAP-kinases in islets immediately following isolation. During the first 48 h, activity of JNK1 and JNK2 declined markedly. Activity of p38 increased steadily with time in culture while extracellular signal regulated kinase (ERK) activity declined dramatically within 24 h post-isolation. High p38 activation relative to ERK activation immediately following isolation correlated with a decrease in islet survival after 36 h in culture. Absence and/or transiency of ERK signaling in conjunction with sustained activation of p38 pathway could be an important regulator of cell death in islets during and following their isolation by commonly employed procedures. Copyright (C) 1999 Federation of European Biochemical Societies.

KW - Apoptosis

KW - Extracellular signal regulated kinase

KW - Islets of Langerhans

KW - JNK

KW - Mitogen activated protein kinase

KW - p38

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Activation and expression of ERK, JNK, and p38 MAP-kinases in isolated islets of Langerhans: Implications for cultured islet survival

Abstract

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Keywords

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