Activated neutrophils induce erythrocyte immunoglobulin binding and membrane protein degradation

D. J. Weiss, Michael P Murtaugh

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Neutrophil-induced alterations in feline erythrocytes were investigated to elucidate the pathogenesis of the erythrocyte destruction associated with inflammatory disease. Incubation of erythrocytes with zymosan-activated neutrophils, at target:effector concentrations of 50:1, resulted in immunoglobulin binding to the erythrocyte membrane and degradation of membrane proteins. Vitamin E and superoxide dismutase/catalase attenuated the immunoglobulin binding. Proteolysis was not prevented by addition of antioxidants or protease inhibitors to the erythrocyte:neutrophil coculture and was not reproduced with chemical oxidation or calcium loading of erythrocytes.

Original languageEnglish (US)
Pages (from-to)438-443
Number of pages6
JournalJournal of Leukocyte Biology
Volume48
Issue number5
DOIs
StatePublished - Jan 1 1990

Keywords

  • erythrocyte membrane
  • erythrocyte proteases
  • neutrophil-induced injury

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