Abstract
Construction of the action potential duration (APD) restitution portrait allows visualization of multiple aspects of the dynamics of periodically paced myocytes at various basic cycle lengths (BCLs). For the first time, we obtained the restitution portrait of isolated rabbit and guinea pig cardiac ventricular myocytes and analyzed the time constant, τ, of APD accommodation and the slopes of different types of restitution curves, Sdyn and S 12, measured at varying BCLs. Our results indicate that both τ and the individual slopes are species and pacing dependent. In contrast, the mutual relationship between slopes Sdyn and S12 does not depend on pacing history, being a generic feature of the species. In addition, the maximum slope S12, measured in the restitution portrait at the lowest BCL, predicts the onset of alternans. Further, we investigated the role of the L-type calcium current, ICa-L, in the restitution portrait. We found that ICa-L dramatically affects APD accommodation, as well as the individual slopes Sdyn and S12 measured in the restitution portrait. However, peak calcium current plays a role only at small values of BCL. In conclusion, the results demonstrate that the restitution portrait is a powerful technique to investigate restitution properties of periodically paced cardiac myocytes and the onset of alternans, in particular. Moreover, the data also show that ICa-L plays a crucial role in multiple aspects of cardiac dynamics measured through the restitution portrait.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2735-2745 |
| Number of pages | 11 |
| Journal | Biophysical journal |
| Volume | 91 |
| Issue number | 7 |
| DOIs | |
| State | Published - Jan 1 2006 |
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Action potential duration restitution portraits of mammalian ventricular myocytes : Role of calcium current. / Tolkacheva, Elena G.; Anumonwo, Justus M.B.; Jalife, José.
In: Biophysical journal, Vol. 91, No. 7, 01.01.2006, p. 2735-2745.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Action potential duration restitution portraits of mammalian ventricular myocytes
T2 - Role of calcium current
AU - Tolkacheva, Elena G.
AU - Anumonwo, Justus M.B.
AU - Jalife, José
PY - 2006/1/1
Y1 - 2006/1/1
N2 - Construction of the action potential duration (APD) restitution portrait allows visualization of multiple aspects of the dynamics of periodically paced myocytes at various basic cycle lengths (BCLs). For the first time, we obtained the restitution portrait of isolated rabbit and guinea pig cardiac ventricular myocytes and analyzed the time constant, τ, of APD accommodation and the slopes of different types of restitution curves, Sdyn and S 12, measured at varying BCLs. Our results indicate that both τ and the individual slopes are species and pacing dependent. In contrast, the mutual relationship between slopes Sdyn and S12 does not depend on pacing history, being a generic feature of the species. In addition, the maximum slope S12, measured in the restitution portrait at the lowest BCL, predicts the onset of alternans. Further, we investigated the role of the L-type calcium current, ICa-L, in the restitution portrait. We found that ICa-L dramatically affects APD accommodation, as well as the individual slopes Sdyn and S12 measured in the restitution portrait. However, peak calcium current plays a role only at small values of BCL. In conclusion, the results demonstrate that the restitution portrait is a powerful technique to investigate restitution properties of periodically paced cardiac myocytes and the onset of alternans, in particular. Moreover, the data also show that ICa-L plays a crucial role in multiple aspects of cardiac dynamics measured through the restitution portrait.
AB - Construction of the action potential duration (APD) restitution portrait allows visualization of multiple aspects of the dynamics of periodically paced myocytes at various basic cycle lengths (BCLs). For the first time, we obtained the restitution portrait of isolated rabbit and guinea pig cardiac ventricular myocytes and analyzed the time constant, τ, of APD accommodation and the slopes of different types of restitution curves, Sdyn and S 12, measured at varying BCLs. Our results indicate that both τ and the individual slopes are species and pacing dependent. In contrast, the mutual relationship between slopes Sdyn and S12 does not depend on pacing history, being a generic feature of the species. In addition, the maximum slope S12, measured in the restitution portrait at the lowest BCL, predicts the onset of alternans. Further, we investigated the role of the L-type calcium current, ICa-L, in the restitution portrait. We found that ICa-L dramatically affects APD accommodation, as well as the individual slopes Sdyn and S12 measured in the restitution portrait. However, peak calcium current plays a role only at small values of BCL. In conclusion, the results demonstrate that the restitution portrait is a powerful technique to investigate restitution properties of periodically paced cardiac myocytes and the onset of alternans, in particular. Moreover, the data also show that ICa-L plays a crucial role in multiple aspects of cardiac dynamics measured through the restitution portrait.
UR - http://www.scopus.com/inward/record.url?scp=33749450902&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33749450902&partnerID=8YFLogxK
U2 - 10.1529/biophysj.106.083865
DO - 10.1529/biophysj.106.083865
M3 - Article
C2 - 16844743
AN - SCOPUS:33749450902
VL - 91
SP - 2735
EP - 2745
JO - Biophysical Journal
JF - Biophysical Journal
SN - 0006-3495
IS - 7
ER -