The G protein-coupled delta opioid receptor gene (dor) is temporally and spatially expressed during development. The DOR receptor plays important roles in diverse biological processes, including pain control, immune functions, and cell survival. We previously found that PI3K/Akt/NF-κB signaling is important in the regulation of dor gene expression during nerve growth factor (NGF)-induced differentiation of PC12h cells, which prompted us to examine whether NF-κB p65 is directly or indirectly involved in the regulation of dor promoter activity. In this study, deletional and functional analysis of the dor promoter revealed a 94-bp NGF-responsive fragment upstream of the dor promoter region and involvement of NF-κB in regulating the promoter activity. Chromatin immunoprecipitation assays demonstrated that NF-κB p65 is directly bound to the dor promoter and such binding is related to NGF/PI3K signaling. Together, the results show that direct association of p65 with the promoter is important in NGF-induced dor promoter activity.
|Original language||English (US)|
|Number of pages||5|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Jan 19 2007|
Bibliographical noteFunding Information:
We are grateful to Dr. Hiroshi Hatanaka for providing the PC12h cell line. We thank Drs. Hsing-Ching Liu and Dimitri Smirnov for providing several seed constructs used in this study. This work was supported by National Institute of Health Grants DA-000546, DA-001583, DA-001806, DA07339, KO5-DA-70554 (HHL), K05-DA00513 (PYL), and by A.&F. Stark Fund of the Minnesota Medical Foundation.
- Delta opioid receptor
- Nerve growth factor
- PC12 cells