Abstract
Mechanical force is needed to mediate endocytosis. Whether actin, the most abundant force-generating molecule, is essential for endocytosis is highly controversial in mammalian cells, particularly synapses, likely due to the use of actin blockers, the efficiency and specificity of which are often unclear in the studied cell. Here we addressed this issue using a knockout approach combined with measurements of membrane capacitance and fission pore conductance, imaging of vesicular protein endocytosis, and electron microscopy. We found that two actin isoforms, β- and γ-actin, are crucial for slow, rapid, bulk, and overshoot endocytosis at large calyx-type synapses, and for slow endocytosis and bulk endocytosis at small hippocampal synapses. Polymerized actin provides mechanical force to form endocytic pits. Actin also facilitates replenishment of the readily releasable vesicle pool, likely via endocytic clearance of active zones. We conclude that polymerized actin provides mechanical force essential for all kinetically distinguishable forms of endocytosis at synapses.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1020-1035 |
| Number of pages | 16 |
| Journal | Neuron |
| Volume | 92 |
| Issue number | 5 |
| DOIs | |
| State | Published - Dec 7 2016 |
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Actin Is Crucial for All Kinetically Distinguishable Forms of Endocytosis at Synapses. / Wu, Xin Sheng; Lee, Sung Hoon; Sheng, Jiansong; Zhang, Zhen; Zhao, Wei Dong; Wang, Dongsheng; Jin, Yinghui; Charnay, Patrick; Ervasti, James M.; Wu, Ling Gang.
In: Neuron, Vol. 92, No. 5, 07.12.2016, p. 1020-1035.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Actin Is Crucial for All Kinetically Distinguishable Forms of Endocytosis at Synapses
AU - Wu, Xin Sheng
AU - Lee, Sung Hoon
AU - Sheng, Jiansong
AU - Zhang, Zhen
AU - Zhao, Wei Dong
AU - Wang, Dongsheng
AU - Jin, Yinghui
AU - Charnay, Patrick
AU - Ervasti, James M.
AU - Wu, Ling Gang
PY - 2016/12/7
Y1 - 2016/12/7
N2 - Mechanical force is needed to mediate endocytosis. Whether actin, the most abundant force-generating molecule, is essential for endocytosis is highly controversial in mammalian cells, particularly synapses, likely due to the use of actin blockers, the efficiency and specificity of which are often unclear in the studied cell. Here we addressed this issue using a knockout approach combined with measurements of membrane capacitance and fission pore conductance, imaging of vesicular protein endocytosis, and electron microscopy. We found that two actin isoforms, β- and γ-actin, are crucial for slow, rapid, bulk, and overshoot endocytosis at large calyx-type synapses, and for slow endocytosis and bulk endocytosis at small hippocampal synapses. Polymerized actin provides mechanical force to form endocytic pits. Actin also facilitates replenishment of the readily releasable vesicle pool, likely via endocytic clearance of active zones. We conclude that polymerized actin provides mechanical force essential for all kinetically distinguishable forms of endocytosis at synapses.
AB - Mechanical force is needed to mediate endocytosis. Whether actin, the most abundant force-generating molecule, is essential for endocytosis is highly controversial in mammalian cells, particularly synapses, likely due to the use of actin blockers, the efficiency and specificity of which are often unclear in the studied cell. Here we addressed this issue using a knockout approach combined with measurements of membrane capacitance and fission pore conductance, imaging of vesicular protein endocytosis, and electron microscopy. We found that two actin isoforms, β- and γ-actin, are crucial for slow, rapid, bulk, and overshoot endocytosis at large calyx-type synapses, and for slow endocytosis and bulk endocytosis at small hippocampal synapses. Polymerized actin provides mechanical force to form endocytic pits. Actin also facilitates replenishment of the readily releasable vesicle pool, likely via endocytic clearance of active zones. We conclude that polymerized actin provides mechanical force essential for all kinetically distinguishable forms of endocytosis at synapses.
UR - http://www.scopus.com/inward/record.url?scp=85003498781&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85003498781&partnerID=8YFLogxK
U2 - 10.1016/j.neuron.2016.10.014
DO - 10.1016/j.neuron.2016.10.014
M3 - Article
C2 - 27840001
AN - SCOPUS:85003498781
VL - 92
SP - 1020
EP - 1035
JO - Neuron
JF - Neuron
SN - 0896-6273
IS - 5
ER -