ActA and human zyxin harbour Arp2/3-independent actin-polymerization activity

Julie Fradelizi, Vincent Noireaux, Julie Plastino, Bernadette Menichi, Daniel Louvard, Cécile Sykes, Roy M. Golsteyn, Evelyne Friederich

Research output: Contribution to journalArticlepeer-review

101 Scopus citations


The actin cytoskeleton is a dynamic network that is composed of a variety of F-actin structures. To understand how these structures are produced, we tested the capacity of proteins to direct actin polymerization in a bead assay in vitro and in a mitochondrial-targeting assay in cells. We found that human zyxin and the related protein ActA of Listeria monocytogenes can generate new actin structures in a vasodilator-stimulated phosphoprotein-dependent (VASP) manner, but independently of the Arp2/3 complex. These results are consistent with the concept that there are multiple actin-polymerization machines in cells. With these simple tests it is possible to probe the specific function of proteins or identify novel molecules that act upon cellular actin polymerization.

Original languageEnglish (US)
Pages (from-to)699-707
Number of pages9
JournalNature Cell Biology
Issue number8
StatePublished - 2001

Bibliographical note

Funding Information:
ACKNOWLEDGEMENTS We thank L. Cabanié for technical support and members of the Louvard laboratory and M. Beckerle for valuable discussions. We also thank P. Chavrier, P. Cossart, E. Gouin, L. Hoffman, C. Koehler and L. Machesky for generously providing reagents. This work was supported by a Curie Fellowship (to R.M.G. and J.P.), a MENRT fellowship (to J.F. and V.N.), and grants from the Association pour la Recherche sur le Cancer and the Centre National de la Recherche Scientifique (France). Correspondence and requests for materials should be addressed to E.F. and R.M.G. Supplementary Information is available on Nature Cell Biology’s website (


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