Acquisition, preparation, and functional assessment of human NK cells for adoptive immunotherapy.

Dean A. Lee, Michael R Verneris, Dario Campana

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Human natural killer (NK) cells, a subset of peripheral blood lymphocytes that lack a T- or B-cell receptor, play a crucial role in the innate immune response to viruses and malignant cells. NK cells differentiate infected or malignant cells from normal cells by a complex balance between activating and inhibitory receptor-ligand interactions. Unlike T cells, NK cells do not proliferate in vitro in response to simple crosslinking of a single activating receptor. While many methods to study T-cell function and phenotype can also be applied to NK cells, this chapter addresses methods that are unique to the preparation and assessment of human NK cells for immunotherapy.

Original languageEnglish (US)
Pages (from-to)61-77
Number of pages17
JournalMethods in molecular biology (Clifton, N.J.)
Volume651
DOIs
StatePublished - Nov 12 2010

Fingerprint

Dive into the research topics of 'Acquisition, preparation, and functional assessment of human NK cells for adoptive immunotherapy.'. Together they form a unique fingerprint.

Cite this