Acidolytic cleavage of tris(alkoxy)benzylamide (PAL) “internal reference” amino acyl (IRAA) anchoring linkages: validation of accepted procedures in solid‐phase peptide synthesis (SPPS)

FERNANDO ALBERICIO, George Barany

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19 Scopus citations

Abstract

Under the normal conditions of acidolytic cleavage/deprotection of tris(a1koxy)benzylamide (PAL) anchoring linkages in Fmoc solid‐phase peptide synthesis (SPPS), product release occurs by a straightforward single‐step pathway. A recently reported cleavage of the NH‐VH bond of an amino acyl residue adjacent to PAL [see Int. J. Peptide Protein Res.38, 146–153 (1991)] could not be confirmed in novel experiments incorporating a double “internal reference” amino acid (IRAA) design. The results of the present work revalidate the widely accepted application of IRAAs to monitor yields in SPPS, and confirm the reliability of PAL methodology for the preparation of C‐terminal peptide amides.

Original languageEnglish (US)
Pages (from-to)307-312
Number of pages6
JournalInternational Journal of Peptide and Protein Research
Volume41
Issue number3
DOIs
StatePublished - Mar 1993

Keywords

  • 5‐(4‐(9‐fluorenylmethyloxycarbonyl)aminomethyl‐3,5‐dimethoxyphenoxy)valeric acid
  • 9‐fluorenylmethyloxycarbonyl
  • C‐terminal peptide amides
  • acidolytic cleavage condition
  • handles
  • solid‐phase peptide synthesis
  • “internal reference” amino acids

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