Acetylshikonin suppressed growth of colorectal tumour tissue and cells by inhibiting the intracellular kinase, T-lymphokine-activated killer cell-originated protein kinase.

Ran Zhao, Bu Young Choi, Lixiao Wei, Mangaladoss Fredimoses, Fanxiang Yin, Xiaorong Fu, Hanyong Chen, Kangdong Liu, Joydeb Kumar Kundu, Zigang Dong, Mee Hyun Lee

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Background and Purpose: Overexpression or aberrant activation of the T-lymphokine-activated killer cell-originated protein kinase (TOPK) promotes gene expression and growth of solid tumours, implying that TOPK would be a rational target in developing novel anticancer drugs. Acetylshikonin, a diterpenoid compound isolated from Lithospermum erythrorhizon root, exerts a range of biological activities. Here we have investigated whether acetylshikonin, by acting as an inhibitor of TOPK, can attenuate the proliferation of colorectal cancer cells and the growth of patient-derived tumours, in vitro and in vivo. Experimental Approach: Targets of acetylshikonin, were identified using kinase profiling analysis, kinetic/binding assay, and computational docking analysis and knock-down techniques. Effects of acetylshikonin on colorectal cancer growth and the underlying mechanisms were evaluated in cell proliferation assays, propidium iodide and annexin-V staining analyses and western blots. Patient-derived tumour xenografts in mice (PDX) and immunohistochemistry were used to assess anti-tumour effects of acetylshikonin. Key Results: Acetylshikonin directly inhibited TOPK activity, interacting with the ATP-binding pocket of TOPK. Acetylshikonin suppressed cell proliferation by inducing cell cycle arrest at the G1 phase, stimulated apoptosis, and increased the expression of apoptotic biomarkers in colorectal cancer cell lines. Mechanistically, acetylshikonin diminished the phosphorylation and activation of TOPK signalling. Furthermore, acetylshikonin decreased the volume of PDX tumours and reduced the expression of TOPK signalling pathway in xenograft tumours. Conclusion and Implications: Acetylshikonin suppressed growth of colorectal cancer cells by attenuating TOPK signalling. Targeted inhibition of TOPK by acetylshikonin might be a promising new approach to the treatment of colorectal cancer.

Original languageEnglish (US)
Pages (from-to)2303-2319
Number of pages17
JournalBritish Journal of Pharmacology
Volume177
Issue number10
DOIs
StatePublished - May 1 2020

Bibliographical note

Funding Information:
We wish to thank Ran Yang, Shen Yang, and Fangfang Liu in the China-US (Henan) Hormel Cancer Institute for supporting experiments. This work was supported by grant funding from the National Natural Science Foundation of China (NSFC81672767 and NSFC81972839) and the Key Program of Henan Province, China Grant 161100510300 and Henan Provincial Government, China.

Funding Information:
We wish to thank Ran Yang, Shen Yang, and Fangfang Liu in the China‐US (Henan) Hormel Cancer Institute for supporting experiments. This work was supported by grant funding from the National Natural Science Foundation of China (NSFC81672767 and NSFC81972839) and the Key Program of Henan Province, China Grant 161100510300 and Henan Provincial Government, China.

Publisher Copyright:
© 2020 The Authors. British Journal of Pharmacology published by John Wiley & Sons Ltd on behalf of British Pharmacological Society

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