Accurate Mass MS/MS/MS Analysis of Siderophores Ferrioxamine B and E1 by Collision-Induced Dissociation Electrospray Mass Spectrometry

Ashley M. Sidebottom, Jonathan A. Karty, Erin E. Carlson

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Siderophores are bacterially secreted, small molecule iron chelators that facilitate the binding of insoluble iron (III) for reuptake and use in various biological processes. These compounds are classified by their iron (III) binding geometry, as dictated by subunit composition and include groups such as the trihydroxamates (hexadentate ligand) and catecholates (bidentate). Small modifications to the core structure such as acetylation, lipid tail addition, or cyclization, make facile characterization of new siderophores difficult by molecular ion detection alone (MS1). We have expanded upon previous fragmentation-directed studies using electrospray ionization collision-induced dissociation tandem mass spectrometry (ESI-CID-MS/MS/MS) and identified diagnostic MS3 features from the trihydroxamate siderophore class for ferrioxamine B and E1 by accurate mass. Diagnostic features for MS3 include C-C, C-N, amide, and oxime cleavage events with proposed losses of water and -CO from the iron (III) coordination sites. These insights will facilitate the discovery of novel trihydroxamate siderophores from complex sample matrices. [Figure not available: see fulltext.]

Original languageEnglish (US)
Pages (from-to)1899-1902
Number of pages4
JournalJournal of the American Society for Mass Spectrometry
Volume26
Issue number11
DOIs
StatePublished - Nov 1 2015

Keywords

  • Accurate mass
  • MS/MS/MS
  • Siderophore
  • Trihydroxamate

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