Accuracy of the Zika IgM antibody capture enzyme-linked immunosorbent assay from the centers for disease control and prevention (CDC Zika MAC-ELISA) for diagnosis of zika virus infection

Moyra Machado Portilho; Laise de Moraes; Mariana Kikuti; Leile Camila Jacob Nascimento; Mitermayer Galvão Reis; Viviane Sampaio Boaventura; Ricardo Khouri; Guilherme Sousa Ribeiro

doi:10.3390/diagnostics10100835

Accuracy of the Zika IgM antibody capture enzyme-linked immunosorbent assay from the centers for disease control and prevention (CDC Zika MAC-ELISA) for diagnosis of zika virus infection

Moyra Machado Portilho
, Laise de Moraes
, Mariana Kikuti
, Leile Camila Jacob Nascimento
, Mitermayer Galvão Reis
, Viviane Sampaio Boaventura
, Ricardo Khouri
, Guilherme Sousa Ribeiro

Veterinary Population Medicine

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Serological diagnosis of Zika virus (ZIKV) infection is challenging because of antigenic cross-reactivity with dengue virus (DENV). This study evaluated the accuracy of the Zika IgM antibody capture enzyme-linked immunosorbent assay (CDC Zika IgM MAC-ELISA) in differentiating between ZIKV and DENV infections. To determine sensitivity, we used acute- and convalescent-phase sera from 21 patients with RT-PCR-confirmed ZIKV infection. To determine specificity, we used acute- and convalescent-phase sera from 60 RT-PCR-confirmed dengue cases and sera from 23 blood donors. During the acute-phase of the illness, the assay presented a sensitivity of 12.5% (2/16) for samples collected 0–4 days post symptoms onset (DPSO), and of 75.0% (3/4) for samples collected 5–9 DPSO. During the convalescent-phase of the illness, the test sensitivity was 90.9% (10/11), 100% (2/2), and 0% (0/2) for samples obtained 12–102, 258–260, and 722–727 DPSO, respectively. Specificity for acute- and convalescent-phase samples from RT-PCR-confirmed dengue cases was 100% and 93.2%, respectively. Specificity for blood donor samples was 100%. The assay is an accurate method for Zika serological diagnosis and proved to be reliable for use during surveillance and outbreak investigations in settings where ZIKV and DENV cocirculate.

Original language	English (US)
Article number	835
Journal	Diagnostics
Volume	10
Issue number	10
DOIs	https://doi.org/10.3390/diagnostics10100835
State	Published - Oct 18 2020

Bibliographical note

Funding Information:
Funding: This study was supported by the Brazilian National Council for Scientific and Technological Development (grant 550160/2010-8 to MGR, grants 400830/2013-2 and 440891/2016-7 to GSR, grant 439967/2016-3 to RK, and scholarships to MGR and GSR); the Bahia Foundation for Research Support (grant PNX0010/2011 to MGR, grants PPP0055/2011, JCB0020/2013, PET0026/2013, APP0044/2016, and PET0022/2016 to GSR); and the Coordination for the Improvement of Higher Education Personnel, Brazilian Ministry of Education (grant 88887.130746/2016-00 to GSR and scholarships to MMP, LM, MK and LCJN).

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

CDC Zika MAC-ELISA
Diagnostic accuracy
Enzyme-linked immunosorbent assay
Sensitivity and specificity
Zika virus

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10.3390/diagnostics10100835

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Portilho, M. M., Moraes, L. D., Kikuti, M., Nascimento, L. C. J., Reis, M. G., Boaventura, V. S., Khouri, R., & Ribeiro, G. S. (2020). Accuracy of the Zika IgM antibody capture enzyme-linked immunosorbent assay from the centers for disease control and prevention (CDC Zika MAC-ELISA) for diagnosis of zika virus infection. Diagnostics, 10(10), Article 835. https://doi.org/10.3390/diagnostics10100835

Accuracy of the Zika IgM antibody capture enzyme-linked immunosorbent assay from the centers for disease control and prevention (CDC Zika MAC-ELISA) for diagnosis of zika virus infection. / Portilho, Moyra Machado; Moraes, Laise de; Kikuti, Mariana et al.
In: Diagnostics, Vol. 10, No. 10, 835, 18.10.2020.

Research output: Contribution to journal › Article › peer-review

Portilho, MM, Moraes, LD, Kikuti, M, Nascimento, LCJ, Reis, MG, Boaventura, VS, Khouri, R & Ribeiro, GS 2020, 'Accuracy of the Zika IgM antibody capture enzyme-linked immunosorbent assay from the centers for disease control and prevention (CDC Zika MAC-ELISA) for diagnosis of zika virus infection', Diagnostics, vol. 10, no. 10, 835. https://doi.org/10.3390/diagnostics10100835

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title = "Accuracy of the Zika IgM antibody capture enzyme-linked immunosorbent assay from the centers for disease control and prevention (CDC Zika MAC-ELISA) for diagnosis of zika virus infection",

abstract = "Serological diagnosis of Zika virus (ZIKV) infection is challenging because of antigenic cross-reactivity with dengue virus (DENV). This study evaluated the accuracy of the Zika IgM antibody capture enzyme-linked immunosorbent assay (CDC Zika IgM MAC-ELISA) in differentiating between ZIKV and DENV infections. To determine sensitivity, we used acute- and convalescent-phase sera from 21 patients with RT-PCR-confirmed ZIKV infection. To determine specificity, we used acute- and convalescent-phase sera from 60 RT-PCR-confirmed dengue cases and sera from 23 blood donors. During the acute-phase of the illness, the assay presented a sensitivity of 12.5\% (2/16) for samples collected 0{\textendash}4 days post symptoms onset (DPSO), and of 75.0\% (3/4) for samples collected 5{\textendash}9 DPSO. During the convalescent-phase of the illness, the test sensitivity was 90.9\% (10/11), 100\% (2/2), and 0\% (0/2) for samples obtained 12{\textendash}102, 258{\textendash}260, and 722{\textendash}727 DPSO, respectively. Specificity for acute- and convalescent-phase samples from RT-PCR-confirmed dengue cases was 100\% and 93.2\%, respectively. Specificity for blood donor samples was 100\%. The assay is an accurate method for Zika serological diagnosis and proved to be reliable for use during surveillance and outbreak investigations in settings where ZIKV and DENV cocirculate.",

keywords = "CDC Zika MAC-ELISA, Diagnostic accuracy, Enzyme-linked immunosorbent assay, Sensitivity and specificity, Zika virus",

author = "Portilho, \{Moyra Machado\} and Moraes, \{Laise de\} and Mariana Kikuti and Nascimento, \{Leile Camila Jacob\} and Reis, \{Mitermayer Galv{\~a}o\} and Boaventura, \{Viviane Sampaio\} and Ricardo Khouri and Ribeiro, \{Guilherme Sousa\}",

note = "Funding Information: Funding: This study was supported by the Brazilian National Council for Scientific and Technological Development (grant 550160/2010-8 to MGR, grants 400830/2013-2 and 440891/2016-7 to GSR, grant 439967/2016-3 to RK, and scholarships to MGR and GSR); the Bahia Foundation for Research Support (grant PNX0010/2011 to MGR, grants PPP0055/2011, JCB0020/2013, PET0026/2013, APP0044/2016, and PET0022/2016 to GSR); and the Coordination for the Improvement of Higher Education Personnel, Brazilian Ministry of Education (grant 88887.130746/2016-00 to GSR and scholarships to MMP, LM, MK and LCJN). Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland.",

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AU - Portilho, Moyra Machado

AU - Moraes, Laise de

AU - Kikuti, Mariana

AU - Nascimento, Leile Camila Jacob

AU - Reis, Mitermayer Galvão

AU - Boaventura, Viviane Sampaio

AU - Khouri, Ricardo

AU - Ribeiro, Guilherme Sousa

N1 - Funding Information: Funding: This study was supported by the Brazilian National Council for Scientific and Technological Development (grant 550160/2010-8 to MGR, grants 400830/2013-2 and 440891/2016-7 to GSR, grant 439967/2016-3 to RK, and scholarships to MGR and GSR); the Bahia Foundation for Research Support (grant PNX0010/2011 to MGR, grants PPP0055/2011, JCB0020/2013, PET0026/2013, APP0044/2016, and PET0022/2016 to GSR); and the Coordination for the Improvement of Higher Education Personnel, Brazilian Ministry of Education (grant 88887.130746/2016-00 to GSR and scholarships to MMP, LM, MK and LCJN). Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2020/10/18

Y1 - 2020/10/18

N2 - Serological diagnosis of Zika virus (ZIKV) infection is challenging because of antigenic cross-reactivity with dengue virus (DENV). This study evaluated the accuracy of the Zika IgM antibody capture enzyme-linked immunosorbent assay (CDC Zika IgM MAC-ELISA) in differentiating between ZIKV and DENV infections. To determine sensitivity, we used acute- and convalescent-phase sera from 21 patients with RT-PCR-confirmed ZIKV infection. To determine specificity, we used acute- and convalescent-phase sera from 60 RT-PCR-confirmed dengue cases and sera from 23 blood donors. During the acute-phase of the illness, the assay presented a sensitivity of 12.5% (2/16) for samples collected 0–4 days post symptoms onset (DPSO), and of 75.0% (3/4) for samples collected 5–9 DPSO. During the convalescent-phase of the illness, the test sensitivity was 90.9% (10/11), 100% (2/2), and 0% (0/2) for samples obtained 12–102, 258–260, and 722–727 DPSO, respectively. Specificity for acute- and convalescent-phase samples from RT-PCR-confirmed dengue cases was 100% and 93.2%, respectively. Specificity for blood donor samples was 100%. The assay is an accurate method for Zika serological diagnosis and proved to be reliable for use during surveillance and outbreak investigations in settings where ZIKV and DENV cocirculate.

AB - Serological diagnosis of Zika virus (ZIKV) infection is challenging because of antigenic cross-reactivity with dengue virus (DENV). This study evaluated the accuracy of the Zika IgM antibody capture enzyme-linked immunosorbent assay (CDC Zika IgM MAC-ELISA) in differentiating between ZIKV and DENV infections. To determine sensitivity, we used acute- and convalescent-phase sera from 21 patients with RT-PCR-confirmed ZIKV infection. To determine specificity, we used acute- and convalescent-phase sera from 60 RT-PCR-confirmed dengue cases and sera from 23 blood donors. During the acute-phase of the illness, the assay presented a sensitivity of 12.5% (2/16) for samples collected 0–4 days post symptoms onset (DPSO), and of 75.0% (3/4) for samples collected 5–9 DPSO. During the convalescent-phase of the illness, the test sensitivity was 90.9% (10/11), 100% (2/2), and 0% (0/2) for samples obtained 12–102, 258–260, and 722–727 DPSO, respectively. Specificity for acute- and convalescent-phase samples from RT-PCR-confirmed dengue cases was 100% and 93.2%, respectively. Specificity for blood donor samples was 100%. The assay is an accurate method for Zika serological diagnosis and proved to be reliable for use during surveillance and outbreak investigations in settings where ZIKV and DENV cocirculate.

KW - CDC Zika MAC-ELISA

KW - Diagnostic accuracy

KW - Enzyme-linked immunosorbent assay

KW - Sensitivity and specificity

KW - Zika virus

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Accuracy of the Zika IgM antibody capture enzyme-linked immunosorbent assay from the centers for disease control and prevention (CDC Zika MAC-ELISA) for diagnosis of zika virus infection

Abstract

Bibliographical note

UN SDGs

Keywords

Publisher link

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