Accuracy of radiographic pixel linear analysis in detecting bone loss in periodontal disease: Study in diabetic rats

Carlos Roberto Emerenciano Bueno; Doris Hissako Sumida; Marco Antônio Hungaro Duarte; Ronald Ordinola-Zapata; Mariane Maffei Azuma; Gustav Guimarães; Tiago Novaes Pinheiro; Luciano Tavares Angelo Cintra

doi:10.1016/j.sdentj.2021.07.004

Accuracy of radiographic pixel linear analysis in detecting bone loss in periodontal disease: Study in diabetic rats

Carlos Roberto Emerenciano Bueno, Doris Hissako Sumida, Marco Antônio Hungaro Duarte, Ronald Ordinola-Zapata, Mariane Maffei Azuma, Gustav Guimarães, Tiago Novaes Pinheiro, Luciano Tavares Angelo Cintra

Endodontics

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1 Scopus citations

Abstract

Introduction: Periodontitis, a complex infectious disease that may lead to irreversible loss of periodontium, is considered a predisposing agent for developing insulin resistance due to the release of inflammatory mediators, showing a bilateral relationship with diabetes mellitus. The investigation of periodontal disease requires a clinical approach and complete intraoral radiographs, even with increasing concerns about radiation exposure. Thus, this study assesses pixel linear analysis accuracy using digital radiography via Digora® in detecting alveolar bone destruction in diabetic rats with periodontal disease.

Methodology: 40 rats were divided into groups (n = 10): control (C), rats with periodontal disease (PD), experimental diabetic rats (ED), experimental diabetic rats with periodontal disease (ED-PD). Diabetes was induced by streptozotocin and periodontal disease by periodontal ligature. After 30 days, maxillae bone destruction was obtained by linear analysis of vertical bone loss using digital radiography and then assessed by micro-CT and histology. Data were analyzed by ANOVA and Tukey's test (p < 0.05).

Results: Radiographic, micro-CT and histological analysis presented accurate and similar results. PD and ED-PD groups showed higher bone destruction than C and ED groups (p < 0.05). Moreover, the ED-PD group had higher bone loss than the PD group (p < 0.05).

Conclusion: The pixel linear analysis via digital radiography was an accurate, low-cost alternative in detecting alveolar bone loss in this rat model. Micro-CT and histological analysis may also be used to obtain linear measures to assess and compare periodontal bone destruction in diabetic rats.

Original language	English (US)
Pages (from-to)	987-996
Number of pages	10
Journal	Saudi Dental Journal
Volume	33
Issue number	8
DOIs	https://doi.org/10.1016/j.sdentj.2021.07.004
State	Published - Dec 2021

Bibliographical note

Funding Information:
This study was supported by a grant (2011/11337-0 and 2010/16999-9) from the Sao Paulo Research Foundation (FAPESP), Sao Paulo, SP, Brazil and by a grant (311650/2018-0) from the Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq), Brazil.

Funding Information:
This study was supported by a grant (2011/11337-0 and 2010/16999-9) from the Sao Paulo Research Foundation ( FAPESP ), Sao Paulo, SP, Brazil and by a grant (311650/2018-0) from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brazil.

Publisher Copyright:
© 2021 The Authors

Keywords

Emission-computed tomography
Experimental diabetes mellitus
Histology
Periodontal disease
Radiography

PubMed: MeSH publication types

Journal Article

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title = "Accuracy of radiographic pixel linear analysis in detecting bone loss in periodontal disease: Study in diabetic rats",

abstract = "Introduction: Periodontitis, a complex infectious disease that may lead to irreversible loss of periodontium, is considered a predisposing agent for developing insulin resistance due to the release of inflammatory mediators, showing a bilateral relationship with diabetes mellitus. The investigation of periodontal disease requires a clinical approach and complete intraoral radiographs, even with increasing concerns about radiation exposure. Thus, this study assesses pixel linear analysis accuracy using digital radiography via Digora{\textregistered} in detecting alveolar bone destruction in diabetic rats with periodontal disease.Methodology: 40 rats were divided into groups (n = 10): control (C), rats with periodontal disease (PD), experimental diabetic rats (ED), experimental diabetic rats with periodontal disease (ED-PD). Diabetes was induced by streptozotocin and periodontal disease by periodontal ligature. After 30 days, maxillae bone destruction was obtained by linear analysis of vertical bone loss using digital radiography and then assessed by micro-CT and histology. Data were analyzed by ANOVA and Tukey's test (p < 0.05).Results: Radiographic, micro-CT and histological analysis presented accurate and similar results. PD and ED-PD groups showed higher bone destruction than C and ED groups (p < 0.05). Moreover, the ED-PD group had higher bone loss than the PD group (p < 0.05).Conclusion: The pixel linear analysis via digital radiography was an accurate, low-cost alternative in detecting alveolar bone loss in this rat model. Micro-CT and histological analysis may also be used to obtain linear measures to assess and compare periodontal bone destruction in diabetic rats.",

keywords = "Emission-computed tomography, Experimental diabetes mellitus, Histology, Periodontal disease, Radiography",

author = "Bueno, {Carlos Roberto Emerenciano} and Sumida, {Doris Hissako} and Duarte, {Marco Ant{\^o}nio Hungaro} and Ronald Ordinola-Zapata and Azuma, {Mariane Maffei} and Gustav Guimar{\~a}es and Pinheiro, {Tiago Novaes} and Cintra, {Luciano Tavares Angelo}",

note = "Funding Information: This study was supported by a grant (2011/11337-0 and 2010/16999-9) from the Sao Paulo Research Foundation (FAPESP), Sao Paulo, SP, Brazil and by a grant (311650/2018-0) from the Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq), Brazil. Funding Information: This study was supported by a grant (2011/11337-0 and 2010/16999-9) from the Sao Paulo Research Foundation ( FAPESP ), Sao Paulo, SP, Brazil and by a grant (311650/2018-0) from the Conselho Nacional de Desenvolvimento Cient{\'i}fico e Tecnol{\'o}gico (CNPq), Brazil. Publisher Copyright: {\textcopyright} 2021 The Authors",

year = "2021",

month = dec,

doi = "10.1016/j.sdentj.2021.07.004",

language = "English (US)",

volume = "33",

pages = "987--996",

journal = "Saudi Dental Journal",

issn = "1013-9052",

publisher = "King Saud University",

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TY - JOUR

T1 - Accuracy of radiographic pixel linear analysis in detecting bone loss in periodontal disease

T2 - Study in diabetic rats

AU - Bueno, Carlos Roberto Emerenciano

AU - Sumida, Doris Hissako

AU - Duarte, Marco Antônio Hungaro

AU - Ordinola-Zapata, Ronald

AU - Azuma, Mariane Maffei

AU - Guimarães, Gustav

AU - Pinheiro, Tiago Novaes

AU - Cintra, Luciano Tavares Angelo

N1 - Funding Information: This study was supported by a grant (2011/11337-0 and 2010/16999-9) from the Sao Paulo Research Foundation (FAPESP), Sao Paulo, SP, Brazil and by a grant (311650/2018-0) from the Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq), Brazil. Funding Information: This study was supported by a grant (2011/11337-0 and 2010/16999-9) from the Sao Paulo Research Foundation ( FAPESP ), Sao Paulo, SP, Brazil and by a grant (311650/2018-0) from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brazil. Publisher Copyright: © 2021 The Authors

PY - 2021/12

Y1 - 2021/12

N2 - Introduction: Periodontitis, a complex infectious disease that may lead to irreversible loss of periodontium, is considered a predisposing agent for developing insulin resistance due to the release of inflammatory mediators, showing a bilateral relationship with diabetes mellitus. The investigation of periodontal disease requires a clinical approach and complete intraoral radiographs, even with increasing concerns about radiation exposure. Thus, this study assesses pixel linear analysis accuracy using digital radiography via Digora® in detecting alveolar bone destruction in diabetic rats with periodontal disease.Methodology: 40 rats were divided into groups (n = 10): control (C), rats with periodontal disease (PD), experimental diabetic rats (ED), experimental diabetic rats with periodontal disease (ED-PD). Diabetes was induced by streptozotocin and periodontal disease by periodontal ligature. After 30 days, maxillae bone destruction was obtained by linear analysis of vertical bone loss using digital radiography and then assessed by micro-CT and histology. Data were analyzed by ANOVA and Tukey's test (p < 0.05).Results: Radiographic, micro-CT and histological analysis presented accurate and similar results. PD and ED-PD groups showed higher bone destruction than C and ED groups (p < 0.05). Moreover, the ED-PD group had higher bone loss than the PD group (p < 0.05).Conclusion: The pixel linear analysis via digital radiography was an accurate, low-cost alternative in detecting alveolar bone loss in this rat model. Micro-CT and histological analysis may also be used to obtain linear measures to assess and compare periodontal bone destruction in diabetic rats.

AB - Introduction: Periodontitis, a complex infectious disease that may lead to irreversible loss of periodontium, is considered a predisposing agent for developing insulin resistance due to the release of inflammatory mediators, showing a bilateral relationship with diabetes mellitus. The investigation of periodontal disease requires a clinical approach and complete intraoral radiographs, even with increasing concerns about radiation exposure. Thus, this study assesses pixel linear analysis accuracy using digital radiography via Digora® in detecting alveolar bone destruction in diabetic rats with periodontal disease.Methodology: 40 rats were divided into groups (n = 10): control (C), rats with periodontal disease (PD), experimental diabetic rats (ED), experimental diabetic rats with periodontal disease (ED-PD). Diabetes was induced by streptozotocin and periodontal disease by periodontal ligature. After 30 days, maxillae bone destruction was obtained by linear analysis of vertical bone loss using digital radiography and then assessed by micro-CT and histology. Data were analyzed by ANOVA and Tukey's test (p < 0.05).Results: Radiographic, micro-CT and histological analysis presented accurate and similar results. PD and ED-PD groups showed higher bone destruction than C and ED groups (p < 0.05). Moreover, the ED-PD group had higher bone loss than the PD group (p < 0.05).Conclusion: The pixel linear analysis via digital radiography was an accurate, low-cost alternative in detecting alveolar bone loss in this rat model. Micro-CT and histological analysis may also be used to obtain linear measures to assess and compare periodontal bone destruction in diabetic rats.

KW - Emission-computed tomography

KW - Experimental diabetes mellitus

KW - Histology

KW - Periodontal disease

KW - Radiography

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JO - Saudi Dental Journal

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ER -

Accuracy of radiographic pixel linear analysis in detecting bone loss in periodontal disease: Study in diabetic rats

Abstract

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