Accuracy of herpes simplex virus detection in liquid-based (SurePath) Papanicolaou tests: A comparison with polymerase chain reaction

Deniz L. Aslan, Stefan E. Pambuccian, Frances L. Prekker, Timothy W Schacker, Peter Southern, Kay Savik, Dan McKeon, H. Evin Gulbahce

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

A review of our institution's Papanicolaou test records over an 11-yr period showed that liquid-based Papanicolaou tests (LBPTs) had a significantly higher frequency of diagnoses of Herpes simplex virus (HSV)-related cellular changes compared to conventional Papanicolaou smears (77/302,841, 0.026% vs. 56/376,173, 0.015%, P = 0.002). To investigate the accuracy of the diagnosis of HSV by LBPT, we performed conventional polymerase chain reaction (PCR) on the residual samples from 258 prospectively collected LBPT and real-time PCR using a different primer set on a subset of 40 LBPT. Conventional PCR was positive in 22 of 22 cases diagnosed of HSV, 1 of 2 cases diagnosed as suspicious for HSV, and none of 234 LBPT without a cytologic HSV diagnosis. Real-time PCR was positive in 8 of 8 cases diagnosed as HSV and none of the 32 controls. We conclude that LBPT allows an increased detection of HSV that is highly accurate.

Original languageEnglish (US)
Pages (from-to)94-103
Number of pages10
JournalDiagnostic Cytopathology
Volume36
Issue number2
DOIs
StatePublished - Feb 1 2008

Keywords

  • Herpes simplex virus
  • Liquid-based Papanicolaou tests
  • PCR
  • Papanicolaou tests
  • Real-time PCR
  • Sensitivity and specificity

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