Accumulation of nitrotyrosine on the SERCA2a isoform of SR Ca-ATPase of rat skeletal muscle during aging: A peroxynitrite-mediated process?

Rosa I. Viner; Deborah A. Ferrington; Andreas F.R. Hühmer; Diana J. Bigelow; Christian Schöneich

doi:10.1016/0014-5793(95)01530-2

Accumulation of nitrotyrosine on the SERCA2a isoform of SR Ca-ATPase of rat skeletal muscle during aging: A peroxynitrite-mediated process?

Rosa I. Viner, Deborah A. Ferrington, Andreas F.R. Hühmer, Diana J. Bigelow, Christian Schöneich

Ophthalmology & Visual Neurosciences

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Abstract

The SR Ca-ATPase in skeletal muscle SR vesicles isolated from young adult (5 months) and aged (28 months) rats was analyzed for nitrotyrosine. Only the SERCA2a isoform contained significant amounts with approximately one and four nitrotyrosine residues per young and old Ca-ATPase, respectively. The in vitro exposure of SR vesicles of young rats to peroxynitrite yielded selective nitration of the SERCA2a Ca-ATPase even in the presence of excess SERCA1a. No nitration was observed during the exposure of SR vesicles to nitric oxide in the presence of O₂. These data suggest the in vivo presence of peroxynitrite in skeletal muscle. The greater nitrotyrosine content of SERCA2a from aged tissue implies an age-associated increase in susceptibility to oxidation by this species.

Original language	English (US)
Pages (from-to)	286-290
Number of pages	5
Journal	FEBS Letters
Volume	379
Issue number	3
DOIs	https://doi.org/10.1016/0014-5793(95)01530-2
State	Published - Feb 5 1996

Keywords

Aging
Nitric oxide
Nitrotyrosine
Peroxynitrite
Reactive oxygen species
SR Ca-ATPase

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10.1016/0014-5793(95)01530-2

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title = "Accumulation of nitrotyrosine on the SERCA2a isoform of SR Ca-ATPase of rat skeletal muscle during aging: A peroxynitrite-mediated process?",

abstract = "The SR Ca-ATPase in skeletal muscle SR vesicles isolated from young adult (5 months) and aged (28 months) rats was analyzed for nitrotyrosine. Only the SERCA2a isoform contained significant amounts with approximately one and four nitrotyrosine residues per young and old Ca-ATPase, respectively. The in vitro exposure of SR vesicles of young rats to peroxynitrite yielded selective nitration of the SERCA2a Ca-ATPase even in the presence of excess SERCA1a. No nitration was observed during the exposure of SR vesicles to nitric oxide in the presence of O2. These data suggest the in vivo presence of peroxynitrite in skeletal muscle. The greater nitrotyrosine content of SERCA2a from aged tissue implies an age-associated increase in susceptibility to oxidation by this species.",

keywords = "Aging, Nitric oxide, Nitrotyrosine, Peroxynitrite, Reactive oxygen species, SR Ca-ATPase",

author = "Viner, {Rosa I.} and Ferrington, {Deborah A.} and H{\"u}hmer, {Andreas F.R.} and Bigelow, {Diana J.} and Christian Sch{\"o}neich",

year = "1996",

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language = "English (US)",

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T1 - Accumulation of nitrotyrosine on the SERCA2a isoform of SR Ca-ATPase of rat skeletal muscle during aging

T2 - A peroxynitrite-mediated process?

AU - Viner, Rosa I.

AU - Ferrington, Deborah A.

AU - Hühmer, Andreas F.R.

AU - Bigelow, Diana J.

AU - Schöneich, Christian

PY - 1996/2/5

Y1 - 1996/2/5

N2 - The SR Ca-ATPase in skeletal muscle SR vesicles isolated from young adult (5 months) and aged (28 months) rats was analyzed for nitrotyrosine. Only the SERCA2a isoform contained significant amounts with approximately one and four nitrotyrosine residues per young and old Ca-ATPase, respectively. The in vitro exposure of SR vesicles of young rats to peroxynitrite yielded selective nitration of the SERCA2a Ca-ATPase even in the presence of excess SERCA1a. No nitration was observed during the exposure of SR vesicles to nitric oxide in the presence of O2. These data suggest the in vivo presence of peroxynitrite in skeletal muscle. The greater nitrotyrosine content of SERCA2a from aged tissue implies an age-associated increase in susceptibility to oxidation by this species.

AB - The SR Ca-ATPase in skeletal muscle SR vesicles isolated from young adult (5 months) and aged (28 months) rats was analyzed for nitrotyrosine. Only the SERCA2a isoform contained significant amounts with approximately one and four nitrotyrosine residues per young and old Ca-ATPase, respectively. The in vitro exposure of SR vesicles of young rats to peroxynitrite yielded selective nitration of the SERCA2a Ca-ATPase even in the presence of excess SERCA1a. No nitration was observed during the exposure of SR vesicles to nitric oxide in the presence of O2. These data suggest the in vivo presence of peroxynitrite in skeletal muscle. The greater nitrotyrosine content of SERCA2a from aged tissue implies an age-associated increase in susceptibility to oxidation by this species.

KW - Aging

KW - Nitric oxide

KW - Nitrotyrosine

KW - Peroxynitrite

KW - Reactive oxygen species

KW - SR Ca-ATPase

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U2 - 10.1016/0014-5793(95)01530-2

DO - 10.1016/0014-5793(95)01530-2

M3 - Article

C2 - 8603707

AN - SCOPUS:0030061132

SN - 0014-5793

VL - 379

SP - 286

EP - 290

JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -

Accumulation of nitrotyrosine on the SERCA2a isoform of SR Ca-ATPase of rat skeletal muscle during aging: A peroxynitrite-mediated process?

Abstract

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