Abundance-dependent proteomic analysis by mass spectrometry

Timothy J. Griffin; Chris M. Lock; Xiao Jun Li; Alpesh Patel; Iryna Chervetsova; Hookeun Lee; Michael E. Wright; Jeffrey A. Ranish; Sharon S. Chen; Ruedi Aebersold

Abundance-dependent proteomic analysis by mass spectrometry

Timothy J. Griffin, Chris M. Lock, Xiao Jun Li, Alpesh Patel, Iryna Chervetsova, Hookeun Lee, Michael E. Wright, Jeffrey A. Ranish, Sharon S. Chen, Ruedi Aebersold

Biochemistry, Molecular Biology, and Biophysics (TMED)

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Scopus citations

Abstract

The technique for the identification and relative quantification of proteins present in two or more complex protein samples was described. The advantages of the given technique include increased efficiency and depth of information in the identification of biologically interesting proteins. The peptides for showing differences in abundance exceeding a user-defined threshold are then selected for identification by MS/MS analysis. It was shown that the approach is useful in profiling studies for the identification of important diagnostic disease markers and the characterization of potential therapeutic targets.

Original language	English (US)
Title of host publication	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Number of pages	1
State	Published - Dec 1 2002
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	6/2/02 → 6/6/02

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title = "Abundance-dependent proteomic analysis by mass spectrometry",

abstract = "The technique for the identification and relative quantification of proteins present in two or more complex protein samples was described. The advantages of the given technique include increased efficiency and depth of information in the identification of biologically interesting proteins. The peptides for showing differences in abundance exceeding a user-defined threshold are then selected for identification by MS/MS analysis. It was shown that the approach is useful in profiling studies for the identification of important diagnostic disease markers and the characterization of potential therapeutic targets.",

author = "Griffin, {Timothy J.} and Lock, {Chris M.} and Li, {Xiao Jun} and Alpesh Patel and Iryna Chervetsova and Hookeun Lee and Wright, {Michael E.} and Ranish, {Jeffrey A.} and Chen, {Sharon S.} and Ruedi Aebersold",

year = "2002",

month = dec,

day = "1",

language = "English (US)",

booktitle = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

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TY - GEN

T1 - Abundance-dependent proteomic analysis by mass spectrometry

AU - Griffin, Timothy J.

AU - Lock, Chris M.

AU - Li, Xiao Jun

AU - Patel, Alpesh

AU - Chervetsova, Iryna

AU - Lee, Hookeun

AU - Wright, Michael E.

AU - Ranish, Jeffrey A.

AU - Chen, Sharon S.

AU - Aebersold, Ruedi

PY - 2002/12/1

Y1 - 2002/12/1

N2 - The technique for the identification and relative quantification of proteins present in two or more complex protein samples was described. The advantages of the given technique include increased efficiency and depth of information in the identification of biologically interesting proteins. The peptides for showing differences in abundance exceeding a user-defined threshold are then selected for identification by MS/MS analysis. It was shown that the approach is useful in profiling studies for the identification of important diagnostic disease markers and the characterization of potential therapeutic targets.

AB - The technique for the identification and relative quantification of proteins present in two or more complex protein samples was described. The advantages of the given technique include increased efficiency and depth of information in the identification of biologically interesting proteins. The peptides for showing differences in abundance exceeding a user-defined threshold are then selected for identification by MS/MS analysis. It was shown that the approach is useful in profiling studies for the identification of important diagnostic disease markers and the characterization of potential therapeutic targets.

UR - http://www.scopus.com/inward/record.url?scp=2442674164&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2442674164&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:2442674164

BT - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Abundance-dependent proteomic analysis by mass spectrometry

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