Abundance-dependent proteomic analysis by mass spectrometry

Timothy J. Griffin, Chris M. Lock, Xiao Jun Li, Alpesh Patel, Iryna Chervetsova, Hookeun Lee, Michael E. Wright, Jeffrey A. Ranish, Sharon S. Chen, Ruedi Aebersold

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The technique for the identification and relative quantification of proteins present in two or more complex protein samples was described. The advantages of the given technique include increased efficiency and depth of information in the identification of biologically interesting proteins. The peptides for showing differences in abundance exceeding a user-defined threshold are then selected for identification by MS/MS analysis. It was shown that the approach is useful in profiling studies for the identification of important diagnostic disease markers and the characterization of potential therapeutic targets.

Original languageEnglish (US)
Title of host publicationProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Number of pages1
StatePublished - Dec 1 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

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Griffin, T. J., Lock, C. M., Li, X. J., Patel, A., Chervetsova, I., Lee, H., Wright, M. E., Ranish, J. A., Chen, S. S., & Aebersold, R. (2002). Abundance-dependent proteomic analysis by mass spectrometry. In Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics