Absolute levels of some free amino acids in normal and biologically fractionated retinas

A. I. Cohen, M. McDaniel, H. Orr

Research output: Contribution to journalArticle

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Abstract

Using isolated (1) normal retinas, (2) receptorless retinas, and (3) retinas with markedly reduced inner layers, respectively, derived from 90 day old pigmented mice were normal, or possessed of a genetic receptor dystrophy, or treated postnatally with monosodium glutamate, extracts were assayed with an animo acid analyzer and/or by fluorometric ultramicro techniques. In millimoles per kilogram protein, the glycine, ananine, and GABA levels were, respectively, 17.0, 4.7, and 18.7 in (1), 38.0, 8.7, and 26.0 in (2), and 9.0, 2.9, and 6.0 in (3). These data suggest a relative concentration of these amino acids in the inner retina with glycine and GABA levels in (2) matching or exceeding published values for any central nervous system region. Taurine, however, had concentrations of 410, 150, and 500 in (1), (2), and (3), respectively, thus suggesting high levels everywhere and a relative concentration in the outer retina. In some groups, glutamate, GABA glutamine, or aspartate levels were lower in receptor containing retinas from dark adapted as compared to light adapted animals. Glutamate concetrations were similar in (1), (2) and (3) and aspartate and glutamine plus serine somewhat elevated in (2). Relative neurophysiologic inactivity and/or the different lesion mechanisms in the two abnormal retinas must affect these data. However, when large concentration differences between (1) and (2) and between (1) and (3) are in opposing directions, true distribution differences in (1) are suggested. The data may bear on amino acids as possible neural transmitters and/or on local metabolic specializations.

Original languageEnglish (US)
Pages (from-to)686-693
Number of pages8
JournalInvestigative Ophthalmology
Volume12
Issue number9
StatePublished - Dec 1 1973

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Retina
Amino Acids
gamma-Aminobutyric Acid
Glutamine
Aspartic Acid
Glycine
Glutamic Acid
Sodium Glutamate
Taurine
Serine
Central Nervous System
Light
Acids
Proteins

Cite this

Absolute levels of some free amino acids in normal and biologically fractionated retinas. / Cohen, A. I.; McDaniel, M.; Orr, H.

In: Investigative Ophthalmology, Vol. 12, No. 9, 01.12.1973, p. 686-693.

Research output: Contribution to journalArticle

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