Absence of drug interaction between Hwang-Ryun-Hae-Dok-Tang and Phenolsulfonphthalein

Hong Jae Yi, Ju Hee Oh, Young Joo Lee

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Hwang-Ryun-Hae-Dok-Tang (HT; a standardized herbal formula consisting of extracts from Coptidis Rhizoma, Scutellariae Radix, Phellodendri Cortex, and Gardeniae Fructus) was reported to modulate a function of multidrug resistance associated protein 2 (Mrp2) in vitro. The aim of this study was to assess the in vivo pharmacokinetic interactions between HT and phenolsulfonphthalein (PSP), a typical model Mrp2 substrate eliminated via bile through Mrp2 in rats. Rats received intravenous PSP (0.8 mg/kg) followed by either a single oral dose of HT (0.42 g/kg) or multiple oral doses of HT (0.42 g/kg for 7 days). The effect of HT treatment was also investigated at a steady-state after intravenous PSP infusion. In contrast to previous in vitro results, in this study, we found that the HT-treated and control groups did not show any significant difference in the plasma PSP concentration and pharmacokinetic parameters, including area under the plasma concentration-time curve (AUC; control: 118 ± 19, single dose: 116 ± 40, and multiple dose: 137 ± 4, in mg/(min•mL)) and biliary clearance (control: 3.15 ± 0.69, single dose: 2.59 ± 1.11, and multiple dose: 2.53 ± 0.65, in mL/(min•kg)). However, cyclosporine A (5 mg/kg, an inhibitor of Mrp2) significantly decreased the AUC and biliary clearance of PSP. The steady-state plasma concentration and biliary clearance of PSP-were also similar between the groups. Taken together, our results suggest that HT may not be affected by Mrp2-mediated herb-drug interaction in vivo.

Original languageEnglish (US)
Pages (from-to)2025-2031
Number of pages7
JournalArchives of Pharmacal Research
Volume33
Issue number12
DOIs
StatePublished - Dec 2010
Externally publishedYes

Keywords

  • Biliary excretion
  • Herb-drug interaction
  • Hwang-Ryun-Hae-Dok-Tang
  • Pharmacokinetics
  • Phenolsulfonphthalein

Fingerprint

Dive into the research topics of 'Absence of drug interaction between Hwang-Ryun-Hae-Dok-Tang and Phenolsulfonphthalein'. Together they form a unique fingerprint.

Cite this