TY - JOUR
T1 - Abnormal centrosome amplification in the absence of p53
AU - Fukasawa, Kenji
AU - Choi, Taesaeng
AU - Kuriyama, Ryoko
AU - Rulong, Shen
AU - Vande Woude, George F.
PY - 1996/3/22
Y1 - 1996/3/22
N2 - The centrosome plays a vital role in mitotic fidelity, ensuring establishment of bipolar spindles and balances chromosome segregation. Centrosome duplication occurs only once during cell cycle and is therefore highly regulated. Here, it is shown that in mouse embryonic fibroblasts (MEFs) lacking the p53 tumor suppressor protein, multiple copies of functinally competent centrosomes are generated during a single cell cycle. In contrast, MEFs prepared from normal mice or mice deficient in the retinoblastoma tumor suppressor gene product do not display these abnormalities. The abnormally amplified centrosomic profoundly affect mitotic fidelity, resulting in unequal segregation of chromosomes. These observations implicate p53 in the regulation of centrosome duplication and suggest one possible mechanism by which the loss of p53 may cause genetic instability.
AB - The centrosome plays a vital role in mitotic fidelity, ensuring establishment of bipolar spindles and balances chromosome segregation. Centrosome duplication occurs only once during cell cycle and is therefore highly regulated. Here, it is shown that in mouse embryonic fibroblasts (MEFs) lacking the p53 tumor suppressor protein, multiple copies of functinally competent centrosomes are generated during a single cell cycle. In contrast, MEFs prepared from normal mice or mice deficient in the retinoblastoma tumor suppressor gene product do not display these abnormalities. The abnormally amplified centrosomic profoundly affect mitotic fidelity, resulting in unequal segregation of chromosomes. These observations implicate p53 in the regulation of centrosome duplication and suggest one possible mechanism by which the loss of p53 may cause genetic instability.
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U2 - 10.1126/science.271.5256.1744
DO - 10.1126/science.271.5256.1744
M3 - Article
C2 - 8596939
AN - SCOPUS:0029881977
SN - 0036-8075
VL - 271
SP - 1744
EP - 1747
JO - Science
JF - Science
IS - 5256
ER -