AAV-mediated gene delivery to the spinal cord by intrathecal injection

Cristina D. Peterson, Alexander G.J. Skorput, Kelley F Kitto, George L Wilcox, Lucy Vulchanova, Carolyn A Fairbanks

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Gene therapy targeting the spinal cord is an important tool for analyzing mechanisms of nervous system diseases and the development of gene therapies. Analogous to a lumbar puncture in humans, the rodent spinal cord can be accessed through an efficient, noninvasive injection. Here we describe a method for AAV-mediated gene transfer to cells of the spinal cord by intrathecal injection of small quantities of AAV vector.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages199-207
Number of pages9
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1950
ISSN (Print)1064-3745

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Keywords

  • Adeno-associated
  • Dorsal root ganglion
  • Spinal cord
  • Viral vector

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

Cite this

Peterson, C. D., Skorput, A. G. J., Kitto, K. F., Wilcox, G. L., Vulchanova, L., & Fairbanks, C. A. (2019). AAV-mediated gene delivery to the spinal cord by intrathecal injection. In Methods in Molecular Biology (pp. 199-207). (Methods in Molecular Biology; Vol. 1950). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9139-6_11