AAV-mediated gene delivery to the spinal cord by intrathecal injection

Cristina D. Peterson, Alexander G.J. Skorput, Kelley F Kitto, George L Wilcox, Lucy Vulchanova, Carolyn A Fairbanks

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Gene therapy targeting the spinal cord is an important tool for analyzing mechanisms of nervous system diseases and the development of gene therapies. Analogous to a lumbar puncture in humans, the rodent spinal cord can be accessed through an efficient, noninvasive injection. Here we describe a method for AAV-mediated gene transfer to cells of the spinal cord by intrathecal injection of small quantities of AAV vector.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages199-207
Number of pages9
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1950
ISSN (Print)1064-3745

Fingerprint

Spinal Injections
Spinal Cord
Genetic Therapy
Genes
Spinal Puncture
Gene Targeting
Nervous System Diseases
Rodentia
Injections

Keywords

  • Adeno-associated
  • Dorsal root ganglion
  • Spinal cord
  • Viral vector

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

Cite this

Peterson, C. D., Skorput, A. G. J., Kitto, K. F., Wilcox, G. L., Vulchanova, L., & Fairbanks, C. A. (2019). AAV-mediated gene delivery to the spinal cord by intrathecal injection. In Methods in Molecular Biology (pp. 199-207). (Methods in Molecular Biology; Vol. 1950). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9139-6_11

AAV-mediated gene delivery to the spinal cord by intrathecal injection. / Peterson, Cristina D.; Skorput, Alexander G.J.; Kitto, Kelley F; Wilcox, George L; Vulchanova, Lucy; Fairbanks, Carolyn A.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 199-207 (Methods in Molecular Biology; Vol. 1950).

Research output: Chapter in Book/Report/Conference proceedingChapter

Peterson, CD, Skorput, AGJ, Kitto, KF, Wilcox, GL, Vulchanova, L & Fairbanks, CA 2019, AAV-mediated gene delivery to the spinal cord by intrathecal injection. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1950, Humana Press Inc., pp. 199-207. https://doi.org/10.1007/978-1-4939-9139-6_11
Peterson CD, Skorput AGJ, Kitto KF, Wilcox GL, Vulchanova L, Fairbanks CA. AAV-mediated gene delivery to the spinal cord by intrathecal injection. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 199-207. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9139-6_11
Peterson, Cristina D. ; Skorput, Alexander G.J. ; Kitto, Kelley F ; Wilcox, George L ; Vulchanova, Lucy ; Fairbanks, Carolyn A. / AAV-mediated gene delivery to the spinal cord by intrathecal injection. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 199-207 (Methods in Molecular Biology).
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