AAV-mediated gene delivery to the spinal cord by intrathecal injection

Cristina D. Peterson; Alexander G.J. Skorput; Kelley F Kitto; George L Wilcox; Lucy Vulchanova; Carolyn A Fairbanks

doi:10.1007/978-1-4939-9139-6_11

AAV-mediated gene delivery to the spinal cord by intrathecal injection

Cristina D. Peterson, Alexander G.J. Skorput, Kelley F Kitto, George L Wilcox, Lucy Vulchanova, Carolyn A Fairbanks

Research output: Chapter in Book/Report/Conference proceeding › Chapter

4 Scopus citations

Abstract

Gene therapy targeting the spinal cord is an important tool for analyzing mechanisms of nervous system diseases and the development of gene therapies. Analogous to a lumbar puncture in humans, the rodent spinal cord can be accessed through an efficient, noninvasive injection. Here we describe a method for AAV-mediated gene transfer to cells of the spinal cord by intrathecal injection of small quantities of AAV vector.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	199-207
Number of pages	9
Volume	1950
DOIs	https://doi.org/10.1007/978-1-4939-9139-6_11
State	Published - 2019

Publication series

Name	Methods in Molecular Biology
Volume	1950
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2019.

Keywords

Adeno-associated
Dorsal root ganglion
Spinal cord
Viral vector
Immunohistochemistry
Injections, Spinal
Gene Transfer Techniques
Gene Expression
Transduction, Genetic
Rats
Spinal Cord/metabolism
Male
Ganglia, Spinal/metabolism
Dependovirus/genetics
Animals
Genetic Vectors/administration & dosage
Fluorescent Antibody Technique
Female
Mice
Transgenes
Genes, Reporter

PubMed: MeSH publication types

Research Support, U.S. Gov't, Non-P.H.S.
Journal Article
Research Support, N.I.H., Extramural

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10.1007/978-1-4939-9139-6_11

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abstract = "Gene therapy targeting the spinal cord is an important tool for analyzing mechanisms of nervous system diseases and the development of gene therapies. Analogous to a lumbar puncture in humans, the rodent spinal cord can be accessed through an efficient, noninvasive injection. Here we describe a method for AAV-mediated gene transfer to cells of the spinal cord by intrathecal injection of small quantities of AAV vector.",

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author = "Peterson, {Cristina D.} and Skorput, {Alexander G.J.} and Kitto, {Kelley F} and Wilcox, {George L} and Lucy Vulchanova and Fairbanks, {Carolyn A}",

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doi = "10.1007/978-1-4939-9139-6_11",

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AU - Peterson, Cristina D.

AU - Skorput, Alexander G.J.

AU - Kitto, Kelley F

AU - Wilcox, George L

AU - Vulchanova, Lucy

AU - Fairbanks, Carolyn A

N1 - Publisher Copyright: © Springer Science+Business Media, LLC, part of Springer Nature 2019.

PY - 2019

Y1 - 2019

N2 - Gene therapy targeting the spinal cord is an important tool for analyzing mechanisms of nervous system diseases and the development of gene therapies. Analogous to a lumbar puncture in humans, the rodent spinal cord can be accessed through an efficient, noninvasive injection. Here we describe a method for AAV-mediated gene transfer to cells of the spinal cord by intrathecal injection of small quantities of AAV vector.

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KW - Dorsal root ganglion

KW - Spinal cord

KW - Viral vector

KW - Immunohistochemistry

KW - Injections, Spinal

KW - Gene Transfer Techniques

KW - Gene Expression

KW - Transduction, Genetic

KW - Rats

KW - Spinal Cord/metabolism

KW - Male

KW - Ganglia, Spinal/metabolism

KW - Dependovirus/genetics

KW - Animals

KW - Genetic Vectors/administration & dosage

KW - Fluorescent Antibody Technique

KW - Female

KW - Mice

KW - Transgenes

KW - Genes, Reporter

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VL - 1950

T3 - Methods in Molecular Biology

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BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

AAV-mediated gene delivery to the spinal cord by intrathecal injection

Abstract

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Bibliographical note

Keywords

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