AAV-mediated gene delivery to the spinal cord by intrathecal injection

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations


Gene therapy targeting the spinal cord is an important tool for analyzing mechanisms of nervous system diseases and the development of gene therapies. Analogous to a lumbar puncture in humans, the rodent spinal cord can be accessed through an efficient, noninvasive injection. Here we describe a method for AAV-mediated gene transfer to cells of the spinal cord by intrathecal injection of small quantities of AAV vector.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages9
StatePublished - 2019

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2019.


  • Adeno-associated
  • Dorsal root ganglion
  • Spinal cord
  • Viral vector
  • Immunohistochemistry
  • Injections, Spinal
  • Gene Transfer Techniques
  • Gene Expression
  • Transduction, Genetic
  • Rats
  • Spinal Cord/metabolism
  • Male
  • Ganglia, Spinal/metabolism
  • Dependovirus/genetics
  • Animals
  • Genetic Vectors/administration & dosage
  • Fluorescent Antibody Technique
  • Female
  • Mice
  • Transgenes
  • Genes, Reporter

PubMed: MeSH publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Journal Article
  • Research Support, N.I.H., Extramural


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