AAV-mediated gene delivery to the enteric nervous system by intracolonic injection

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

The enteric nervous system of the lower gastrointestinal tract comprises intrinsic neural circuits as well as extrinsic afferent and efferent innervation. The development of strategies for neuronal gene transfer has created new opportunities for functional analysis, circuit mapping, and neuromodulation in the enteric nervous system. Studies of AAV-mediated gene transfer to enteric neurons and dorsal root ganglion neurons (DRG) have provided proofs-of-concept for the utility of AAV vectors for genetic manipulations of the intrinsic and extrinsic components of the enteric nervous system. Here we describe a method for AAV-mediated gene transfer to enteric neurons of the descending colon as well as colon-innervating DRG neurons by injection within the intestinal wall (intracolonic injection).

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages407-415
Number of pages9
DOIs
StatePublished - 2019

Publication series

NameMethods in Molecular Biology
Volume1950
ISSN (Print)1064-3745

Bibliographical note

Funding Information:
This work was supported by NIH-SPARC U18EB021716 and T32DA007234.

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2019.

Keywords

  • Colon
  • Dorsal root ganglion
  • Enteric
  • Viral vector
  • Gene Transfer Techniques
  • Gene Expression
  • Transduction, Genetic
  • Enteric Nervous System/metabolism
  • Male
  • Dependovirus/genetics
  • Animals
  • Genetic Vectors/administration & dosage
  • Female
  • Mice
  • Transgenes
  • Genes, Reporter

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural

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