AAL Toxins, funionisms (biology and chemistry) and host-specificity concepts

Chester J. Mirocha, David G. Gilchrist, W. T. Shier, H. K. Abbas, Y. Wen, Ronald F. Vesonder

Research output: Contribution to journalArticlepeer-review

59 Scopus citations

Abstract

The AAL toxins and the fumonisins (FB1 and FB2) are structurally related and produced respectively by Alternaria alternata f.sp. lycopersici and Fusarium moniliforme. AAL toxin is characterized as a hostspecific toxin, toxic to tomato, whereas fumonisin B1 causes equine leukoencephalomalacia. FB1 and FB2 were biologically active in susceptible tomato tissue (Earlypak-7) and animal tissue culture (rat hepatoma H4TG and dog kidney MDCK). Conversely, AAL toxin was also active in the rat and dog tissue culture cells. Both fungi produce toxin/s in culture that cause death in rats; these toxins are other than AAL and fumonisin. The peracetylated derivatives of AAL and FB1 are biologically inactive in both the tomato bioassay and the animal tissue culture systems. Acetylation of the amine renders AAL inactive. The hydrolysis product of AAL (pentolamine) is toxic to the susceptible tomato line whereas the pentolamine of fumonisin is not. AAL and FB1 can be analyzed by Continuous Flow Fast Atom Bombardment (CFFAB) and Ionspray Mass Spectrometry (ISM), both sensitive to the picomole range. The N-acetyl of the TFA hydrolysis product of AAL and FB1 is determined by comparing the fragment ions at m/z 86 and 140 for FB1 and 72 and 126 for AAL.

Original languageEnglish (US)
Pages (from-to)47-56
Number of pages10
JournalMycopathologia
Volume117
Issue number1-2
DOIs
StatePublished - Feb 1 1992

Keywords

  • AAL toxin
  • Alternaria alternata f.sp. lycopersici
  • Fumonisin
  • Fusarium moniliforme
  • host-specific

Fingerprint Dive into the research topics of 'AAL Toxins, funionisms (biology and chemistry) and host-specificity concepts'. Together they form a unique fingerprint.

Cite this