TRAIL is a member of the tumor necrosis factor (TNF) superfamily. This cytokine is cytotoxic for a high proportion of tumor cells, but could be also toxic for normal cells. There is a need to find other agents able to potentiate the antitumor effects of this cytokine. In our study, we found that Ala-Ala-Phe-chloromethylketone (AAF-cmk) augmented cytotoxic activity of TRAIL or TNF against human leukemic cells. Flow cytometry studies and electron microscopy revealed that apoptosis was primarily responsible for this potentiation. Altogether, our studies indicate that AAF-cmk might effectively sensitize human leukemia cells to apoptosis induced by TRAIL and TNF.
Bibliographical noteFunding Information:
This work has been supported by KBN Grant 4 P05A 084 16 to CW, by the Medical University Grant 01-1M15-NS2-2002 to IM and by the CMKP grant 501-2-1-03-48/02 to GH.
- Tripeptidylpeptidase II