AAF-cmk sensitizes tumor cells to trail-mediated apoptosis

Izabela Młnarczuk, Paweł Mróz, Grazyna Hoser, Dominika Nowis, Łukasz P. Biały, Halina Ziemba, Tomasz Grzela, Wojciech Feleszko, Jacek Malejczyk, Cezary Wójcik, Marek Jakóbisiak, Jakub Goła̧b

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

TRAIL is a member of the tumor necrosis factor (TNF) superfamily. This cytokine is cytotoxic for a high proportion of tumor cells, but could be also toxic for normal cells. There is a need to find other agents able to potentiate the antitumor effects of this cytokine. In our study, we found that Ala-Ala-Phe-chloromethylketone (AAF-cmk) augmented cytotoxic activity of TRAIL or TNF against human leukemic cells. Flow cytometry studies and electron microscopy revealed that apoptosis was primarily responsible for this potentiation. Altogether, our studies indicate that AAF-cmk might effectively sensitize human leukemia cells to apoptosis induced by TRAIL and TNF.

Original languageEnglish (US)
Pages (from-to)53-61
Number of pages9
JournalLeukemia research
Volume28
Issue number1
DOIs
StatePublished - Jan 2004
Externally publishedYes

Bibliographical note

Funding Information:
This work has been supported by KBN Grant 4 P05A 084 16 to CW, by the Medical University Grant 01-1M15-NS2-2002 to IM and by the CMKP grant 501-2-1-03-48/02 to GH.

Keywords

  • AAF-cmk
  • Apoptosis
  • Lymphoma
  • TNF
  • TRAIL
  • Tripeptidylpeptidase II

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