A whole-genome shotgun approach for assembling and anchoring the hexaploid bread wheat genome

Jarrod A. Chapman, Martin Mascher, Aydin Buluç, Kerrie Barry, Evangelos Georganas, Adam Session, Veronika Strnadova, Jerry Jenkins, Sunish Sehgal, Leonid Oliker, Jeremy Schmutz, Katherine A. Yelick, Uwe Scholz, Robbie Waugh, Jesse A. Poland, Gary J. Muehlbauer, Nils Stein, Daniel S. Rokhsar

Research output: Contribution to journalArticlepeer-review

161 Scopus citations

Abstract

Polyploid species have long been thought to be recalcitrant to whole-genome assembly. By combining high-throughput sequencing, recent developments in parallel computing, and genetic mapping, we derive, de novo, a sequence assembly representing 9.1 Gbp of the highly repetitive 16 Gbp genome of hexaploid wheat, Triticum aestivum, and assign 7.1 Gb of this assembly to chromosomal locations. The genome representation and accuracy of our assembly is comparable or even exceeds that of a chromosome-by-chromosome shotgun assembly. Our assembly and mapping strategy uses only short read sequencing technology and is applicable to any species where it is possible to construct a mapping population.

Original languageEnglish (US)
Article number26
JournalGenome biology
Volume16
Issue number1
DOIs
StatePublished - Jan 31 2015

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