A volatile liquid chromatography system for nucleotides

John T. Axelxon, James W. Bodley, Timothy F Walseth

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

A liquid chromatography system useful for the rapid purification of radioactively labeled and nonradioactive ribonucleoside mono-, di-, and triphosphates has been developed. The system employs the strong anion-exchange resin AG MP-1 and low concentrations of the volatile eluting agent trifluoroacetic acid, and is suitable for use with high-performance liquid chromatography systems in either isocratic or gradient elution modes. Most common nucleotides can be completely resolved and nearly quantitatively recovered following solvent volatilization.

Original languageEnglish (US)
Pages (from-to)357-360
Number of pages4
JournalAnalytical Biochemistry
Volume116
Issue number2
DOIs
StatePublished - Sep 15 1981

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