A visible fluorescent nanovaccine based on functional genipin crosslinked ovalbumin protein nanoparticles

Xia Dong, Zhiting Sun, Jie Liang, Hai Wang, Dunwan Zhu, Xigang Leng, Chun Wang, Deling Kong, Feng Lv

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Accurate and efficient antigen delivery is crucial for inducing a strong and long-term immune response. A visible protein nanovaccine made from antigen could provide a novel and promising technology for secure and efficient delivery of the antigen with imaging visualization. In this study, a functional nanovaccine based on genipin crosslinked ovalbumin (OVA) fluorescent nanoparticles with chitosan (CS-OVA-NPs) was developed. The nanovaccine can carry abundant antigens by self-crosslinking without additional carriers. The fluorescence imaging technique was applied to monitor and reveal the process of antigen delivery in vivo based on the fluorescence of genipin with a non-invasive and real-time manner. This functional OVA nanovaccine can enhance the uptake of OVA in Dendritic Cells (DCs) and further promote DCs to maturate in vitro. In vivo study further indicated CS-OVA-NPs could trigger antigen-specific immune responses, which demonstrated that this fluorescent nanovaccine provided a novel design approach for accurate and efficient vaccine delivery.

Original languageEnglish (US)
Pages (from-to)1087-1098
Number of pages12
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume14
Issue number4
DOIs
StatePublished - Jun 2018

Bibliographical note

Funding Information:
This research was supported by the National Natural Science Foundation of China (Nos. 81601595, 31200732), the Natural Science Foundation of Tianjin, China (Nos. 16JCYBJC27800, 14JCYBJC17400), the Science and Technology Support Program of Tianjin (14RCGFSY00146) and the CAMS Innovation Fund for Medical Sciences (CIFMS 2017-I2M-3-020). The authors report no conflicts of interest in this work.

Publisher Copyright:
© 2018 Elsevier Inc.

Keywords

  • Antigen delivery
  • In vivo fluorescence tracking
  • Nanovaccine
  • Ovalbumin
  • Protein nanoparticles

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