A viral genome packaging motor transitions between cyclic and helical symmetry to translocate dsDNA

Michael Woodson, Joshua Pajak, Bryon P. Mahler, Wei Zhao, Wei Zhang, Gaurav Arya, Mark A. White, Paul J. Jardine, Marc C. Morais

Research output: Contribution to journalArticlepeer-review

Abstract

Molecular segregation and biopolymer manipulation require the action of molecular motors to do work by applying directional forces to macromolecules. The additional strand conserved E (ASCE) ring motors are an ancient family of molecular motors responsible for diverse biological polymer manipulation tasks. Viruses use ASCE segregation motors to package their genomes into their protein capsids and provide accessible experimental systems due to their relative simplicity. We show by cryo-EM-focused image reconstruction that ASCE ATPases in viral double-stranded DNA (dsDNA) packaging motors adopt helical symmetry complementary to their dsDNA substrates. Together with previous data, our results suggest that these motors cycle between helical and planar configurations, providing a possible mechanism for directional translocation of DNA. Similar changes in quaternary structure have been observed for proteasome and helicase motors, suggesting an ancient and common mechanism of force generation that has been adapted for specific tasks over the course of evolution.

Original languageEnglish (US)
Article numbereabc1955
JournalScience Advances
Volume7
Issue number19
DOIs
StatePublished - May 2021

Bibliographical note

Funding Information:
This work was supported by NIH public health service grants GM122979 (to P.J.J. and M.C.M.), GM127365 (to M.C.M.), and GM118817 (to G.A.). We would also like to acknowledge the Sealy Center for Structural Biology and Molecular Biophysics (SCSB) for support of the UTMB cryo-EM and computational core facilities. Cryo-EM data were also collected at NIH-supported regional cryo-EM imaging facilities operated by W. Chiu (1U24 GM116787-01) and by H. Zhou (1U24 GM116792-01).

Publisher Copyright:
Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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